Lgi4 Rat mAb: FAQs for Research Use

Lgi4 Rat mAb is a rat-derived monoclonal antibody designed to specifically recognize the Lgi4 (Leucine-rich Glioma Inactivated 4) protein, supporting a range of standard immunological research applications.

Product SKU

Ab349843

Antibody Type

Rat Monoclonal Antibody (mAb)

Target Protein

Lgi4 (Leucine-rich Glioma Inactivated 4)

Host Species

Rat

Antibody Format

Monoclonal

Supplier Reference

absea#KC-1407

Overview of Lgi4 and Its Biological Significance

Lgi4 (Leucine-rich Glioma Inactivated 4) is a secreted protein belonging to the LGI family, which plays important roles in the development and maintenance of the peripheral nervous system. Lgi4 has been implicated in Schwann cell differentiation and axon myelination, making it a protein of substantial interest in studies of peripheral neuropathy, nerve regeneration, and developmental neurobiology. Monoclonal antibodies against Lgi4 enable researchers to study its expression patterns, localization, and interaction partners with high specificity and reproducibility.

Recommended Applications and Protocols

The Lgi4 Rat mAb (Ab349843) is intended for use in standard immunological research applications. Typical applications for monoclonal antibodies of this class include Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), and Immunoprecipitation (IP). Users should verify the suitability of this antibody for each specific application and optimize conditions such as antibody dilution, blocking buffer, and antigen retrieval method according to their experimental system. Please refer to the product datasheet for confirmed application details and recommended dilution ranges.

For Western Blot, standard reducing SDS-PAGE conditions are generally recommended as a starting point. For IHC on formalin-fixed paraffin-embedded (FFPE) sections, heat-induced epitope retrieval (HIER) with citrate buffer (pH 6.0) or EDTA buffer (pH 9.0) may be required. Optimization is advised for each new sample type.

Storage, Handling, and Stability

To maintain antibody activity and ensure reproducible results, proper storage and handling of the Lgi4 Rat mAb (Ab349843) are essential. As a general guideline for monoclonal antibodies, the product should be stored at –20 °C for long-term storage and at 4 °C for short-term use. Repeated freeze-thaw cycles should be avoided, as they can degrade antibody performance. It is recommended to aliquot the antibody upon receipt to minimize freeze-thaw events. Please consult the official product datasheet for confirmed storage conditions specific to Ab349843.

Troubleshooting Common Issues

Researchers may encounter several common issues when working with the Lgi4 Rat mAb. Below are general troubleshooting recommendations:

• No signal or weak signal: Verify that the antibody concentration and incubation conditions are appropriate. Ensure the target protein is expressed in your sample. Check secondary antibody compatibility (must be anti-rat).
• High background: Increase blocking time or concentration. Optimize antibody dilution. Ensure washing steps are thorough.
• Non-specific bands (Western Blot): Optimize antibody dilution. Consider using a more stringent blocking agent such as 5% BSA instead of milk for phospho-targets or secreted proteins.
• Inconsistent results: Avoid repeated freeze-thaw cycles. Standardize sample preparation and loading.

For application-specific troubleshooting, please contact Aladdin Scientific technical support.

Frequently asked questions

What is the host species of the Lgi4 mAb (Ab349843)?

The Lgi4 mAb (Ab349843) is produced in rat, making it a rat-derived monoclonal antibody. When selecting a secondary antibody, ensure it is directed against rat immunoglobulins (anti-rat secondary antibody).

Which applications is the Lgi4 Rat mAb suitable for?

The Lgi4 Rat mAb (Ab349843) is designed for use in standard immunological research applications. Typical applications for this class of antibody include Western Blot, Immunofluorescence, Immunohistochemistry, and Immunoprecipitation. Please consult the official product datasheet for the confirmed and validated list of applications specific to Ab349843.

How should the Lgi4 Rat mAb be stored to maintain its activity?

As a general best practice for monoclonal antibodies, store Ab349843 at –20 °C for long-term preservation and at 4 °C for short-term use. Avoid repeated freeze-thaw cycles by aliquoting the antibody upon receipt. Always refer to the specific storage conditions stated on the product datasheet.

What secondary antibody should I use with the Lgi4 Rat mAb?

Since the Lgi4 mAb (Ab349843) is a rat-derived antibody, you must use an anti-rat secondary antibody that is conjugated to your detection system of choice (e.g., HRP, fluorophore). Using an anti-mouse or anti-rabbit secondary antibody will not yield a specific signal.

What is the biological role of the Lgi4 protein targeted by this antibody?

Lgi4 (Leucine-rich Glioma Inactivated 4) is a secreted protein involved in peripheral nervous system development, particularly in Schwann cell differentiation and axon myelination. It is a member of the LGI protein family and is studied in contexts of peripheral neuropathy and neurodevelopmental biology.

References

1. Absea Biotechnology product reference: KC-1407. Lgi4 Rat Monoclonal Antibody.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.