NUDT22 Mouse mAb Protocol: Applications & Usage Guide

NUDT22 Mouse mAb is a monoclonal antibody raised in mouse and supplied in PBS, designed for specific detection of the NUDT22 protein in a range of standard immunological assays.

Product Name

NUDT22 Mouse mAb

SKU

Ab351022

Host Species

Mouse

Antibody Format

Monoclonal Antibody (mAb)

Formulation

PBS only

Target Protein

NUDT22 (Nudix Hydrolase 22)

Overview of NUDT22 and Antibody Specifications

NUDT22 (Nudix Hydrolase 22) is a member of the Nudix hydrolase superfamily, a group of enzymes that catalyze the hydrolysis of nucleoside diphosphates linked to other moieties. NUDT22 has been implicated in nucleotide metabolism and cellular homeostasis. The NUDT22 Mouse mAb (SKU: Ab351022) is a monoclonal antibody generated in mouse and formulated in phosphate-buffered saline (PBS) without additional preservatives or stabilizers, as specified. Researchers should verify buffer compatibility with their downstream assay requirements before use.

Key Specifications:

• Host: Mouse
• Format: Monoclonal Antibody
• Formulation: PBS only
• SKU: Ab351022

Sample Preparation

Proper sample preparation is critical for obtaining reliable results with NUDT22 Mouse mAb. Follow the guidelines below depending on your application:

• Cell Lysates: Lyse cells in RIPA buffer supplemented with protease inhibitor cocktail on ice for 30 minutes. Centrifuge at 12,000 × g for 15 minutes at 4°C. Collect supernatant and determine protein concentration by BCA or Bradford assay.
• Tissue Lysates: Homogenize tissue in lysis buffer containing protease inhibitors. Centrifuge and collect supernatant as above.
• Fixed Cells/Tissues (for IHC/ICC): Use standard formalin fixation and paraffin embedding (FFPE) or cryo-sectioning protocols. Perform antigen retrieval using citrate buffer (pH 6.0) or EDTA buffer (pH 9.0) as appropriate.

Note: Specific sample preparation conditions may require optimization depending on the cell or tissue type and the application used.

Recommended Protocol for Western Blot

The following is a general Western blot protocol for use with NUDT22 Mouse mAb (Ab351022). Specific dilutions and incubation conditions should be optimized empirically for each laboratory setting.

1. Gel Electrophoresis: Separate 20–40 µg of total protein per lane by SDS-PAGE using an appropriate percentage gel for the molecular weight of NUDT22.
2. Transfer: Transfer proteins to a PVDF or nitrocellulose membrane using wet or semi-dry transfer methods.
3. Blocking: Block the membrane with 5% non-fat dry milk or 5% BSA in TBST for 1 hour at room temperature.
4. Primary Antibody Incubation: Incubate with NUDT22 Mouse mAb at the recommended dilution (to be determined by titration) in blocking buffer overnight at 4°C.
5. Washing: Wash membrane 3 times with TBST, 10 minutes each wash.
6. Secondary Antibody: Incubate with an appropriate HRP-conjugated anti-mouse secondary antibody for 1 hour at room temperature.
7. Washing: Wash membrane 3 times with TBST, 10 minutes each wash.
8. Detection: Develop using ECL substrate and image using X-ray film or a digital imaging system.

Important: Recommended working dilutions are not specified in the current product documentation. Users should perform a dilution series to determine optimal antibody concentration for their system.

Storage, Handling, and Quality Control

Storage: Store NUDT22 Mouse mAb (Ab351022) at –20°C for long-term storage. For short-term use (up to 1 month), the antibody may be stored at 4°C. Avoid repeated freeze-thaw cycles, as these can reduce antibody activity. Aliquoting is recommended upon first use.

Handling: The antibody is formulated in PBS only. Do not dilute with buffers containing sodium azide or other inhibitory preservatives unless verified compatible with your assay. Centrifuge briefly before opening the vial to collect contents at the bottom.

Quality Control: Each lot of NUDT22 Mouse mAb should be tested by the end user in their specific assay system. It is recommended to include appropriate positive and negative controls in every experiment to confirm antibody specificity and assay validity.

Frequently asked questions

What formulation is NUDT22 Mouse mAb (Ab351022) supplied in?

NUDT22 Mouse mAb (Ab351022) is supplied in PBS (phosphate-buffered saline) only, without additional preservatives or carrier proteins. Users should verify buffer compatibility with their specific assay requirements.

How should NUDT22 Mouse mAb be stored to maintain optimal activity?

For long-term storage, keep the antibody at –20°C. For frequent use within one month, storage at 4°C is acceptable. To preserve activity, avoid repeated freeze-thaw cycles and aliquot the antibody upon first use.

What positive control should be used when testing NUDT22 Mouse mAb?

A cell line or tissue known to express NUDT22 endogenously should be used as a positive control. The specific recommended positive control cell line is not detailed in the current product documentation; users should consult published literature or contact Aladdin Scientific technical support for guidance.

What applications is NUDT22 Mouse mAb validated for?

The current product documentation does not specify validated applications for Ab351022. Common applications for monoclonal antibodies of this type include Western blot (WB), immunohistochemistry (IHC), and immunofluorescence (IF), but users should independently validate the antibody for their intended application and contact Aladdin Scientific for the latest validation data.

References

1. Aladdin Scientific. NUDT22 Mouse mAb, SKU Ab351022. Product datasheet. Aladdin Scientific.
2. Mildvan AS, et al. Structures and mechanisms of Nudix hydrolases. Arch Biochem Biophys. 2005;433(1):129-143.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.