PFKFB2 Mouse mAb: FAQs for Research Use

PFKFB2 Mouse mAb is a mouse monoclonal antibody targeting the PFKFB2 protein, a key bifunctional enzyme that regulates intracellular fructose-2,6-bisphosphate levels and thereby controls glycolytic flux in mammalian cells.

Product Name

PFKFB2 Mouse mAb

SKU

Ab351028

Host Species

Mouse

Antibody Type

Monoclonal Antibody (mAb)

Formulation

PBS only (no carrier protein or preservative indicated)

Reference Source

Absea #KC-46131

Supplier

Aladdin Scientific

What Is PFKFB2 and Why Is It a Research Target?

PFKFB2 (6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2) is a bifunctional enzyme that catalyzes both the synthesis and degradation of fructose-2,6-bisphosphate (F2,6BP), a potent allosteric activator of phosphofructokinase-1 (PFK-1). By controlling intracellular F2,6BP concentrations, PFKFB2 plays a pivotal role in regulating glycolytic flux and energy homeostasis in mammalian cells.

Research interest in PFKFB2 has grown in the context of cancer metabolism, cardiac physiology, and metabolic disease, where altered glycolytic regulation is a key pathological feature. Detecting and quantifying PFKFB2 protein expression is therefore important for studies investigating metabolic reprogramming.

Product Details and Formulation

Aladdin Scientific's PFKFB2 Mouse mAb (SKU: Ab351028) is a mouse-derived monoclonal antibody produced with reference to Absea clone #KC-46131. The antibody is supplied in PBS (phosphate-buffered saline) only, without added carrier proteins such as BSA or preservatives such as sodium azide, unless otherwise stated on the product datasheet.

The PBS-only formulation is advantageous for applications that are sensitive to carrier proteins or preservatives, such as certain conjugation chemistries or functional assays. Researchers should consult the official product datasheet for confirmed concentration, exact buffer composition, and lot-specific quality control data.

Recommended Applications and Usage Guidelines

Mouse monoclonal antibodies against PFKFB2 are commonly employed in a range of immunological and biochemical assays. Typical applications for antibodies of this class include Western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry (IHC), and enzyme-linked immunosorbent assay (ELISA). However, validated applications for Ab351028 specifically should be confirmed by reviewing the product datasheet or contacting Aladdin Scientific technical support.

Optimal working dilutions vary by application and sample type. It is recommended to perform titration experiments using appropriate positive controls (e.g., cell lysates known to express PFKFB2) to determine the best antibody concentration for each experimental system.

Storage, Handling, and Stability

To maintain antibody integrity and activity, PFKFB2 Mouse mAb should be stored according to the conditions specified on the product datasheet. As a general guideline for PBS-formulated monoclonal antibodies without preservatives, short-term storage at 4°C (up to 1 month) and long-term storage at -20°C or -80°C in small aliquots are recommended to avoid repeated freeze-thaw cycles, which can lead to aggregation and loss of binding activity.

Avoid prolonged exposure to room temperature. If the antibody is intended for long-term storage, adding a carrier protein such as BSA (0.1–1%) may be considered after consulting the datasheet to ensure compatibility with downstream applications.

Ordering and Technical Support

PFKFB2 Mouse mAb is available from Aladdin Scientific under SKU Ab351028. To place an order, visit the Aladdin Scientific website, contact your regional sales representative, or reach out to our customer service team. Volume discounts and institutional pricing may be available; please inquire with our sales team for details.

For technical questions regarding experimental optimization, cross-reactivity, or compatibility with specific assay platforms, contact Aladdin Scientific technical support. Our team can provide guidance on dilution conditions, recommended controls, and troubleshooting for common assay issues.

Frequently asked questions

What species does PFKFB2 Mouse mAb (Ab351028) target?

Ab351028 is a mouse monoclonal antibody raised against PFKFB2. The specific target species reactivity (e.g., human, mouse, rat) should be confirmed by consulting the official product datasheet, as cross-reactivity data are lot- and clone-specific.

Why is the PFKFB2 Mouse mAb formulated in PBS only?

The PBS-only formulation (without BSA or sodium azide) offers greater flexibility for downstream applications sensitive to carrier proteins or preservatives, such as antibody conjugation, functional assays, or certain ELISA formats. Researchers who require a stabilizing carrier for long-term storage should consult the datasheet before adding any additives.

What applications is PFKFB2 Mouse mAb validated for?

Validated applications for Ab351028 are specified on the Aladdin Scientific product datasheet. Common applications for monoclonal antibodies of this type include Western blotting, immunoprecipitation, immunofluorescence, and immunohistochemistry. Please refer to the current datasheet or contact technical support for the most up-to-date validation data.

How should I store PFKFB2 Mouse mAb to maintain its activity?

For short-term use (up to one month), storage at 4°C is generally acceptable. For long-term storage, aliquot the antibody and store at -20°C or -80°C to avoid repeated freeze-thaw cycles. Always follow the specific storage instructions provided on the product datasheet.

How do I order Ab351028 and what pricing options are available?

Ab351028 can be ordered directly through the Aladdin Scientific website using the SKU Ab351028, or via your regional sales representative. Volume discounts and institutional pricing may be available; contact the Aladdin Scientific sales team for a customized quote.

References

1. Absea Biotechnology. KC-46131 PFKFB2 Monoclonal Antibody Product Information.
2. Okar DA, Lange AJ. Fructose-2,6-bisphosphate and control of carbohydrate metabolism in eukaryotes. Biofactors. 1999;10(1):1-14.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.