Laboratory Protocols & SOPs Library
- Experiments for the determination of oxygen radical content in plants SummaryA portion of the oxygen molecules in living organisms, when involved in enzymatic or non-enzymatic reactions, are converted to superoxide anion radicals (O2-) if they accept only one electron.O2- can both interact directly with reactive substances in the body, such as proteins and nucleic ...
- Chromosome observation experiments in meiosis of plant pollen mother cells SummaryMeiosis is a specialized type of cell division in which the sex mother cell divides to form gametes. In this process, chromosomes are replicated once and the cell divides twice, resulting in gametes with half the number of chromosomes compared to the mother cell. Fertilization of the male ...
- Experiments for the determination of the permeability of plant cytoplasmic membranes SummaryThe plant cytoplasmic membrane is the interface between the cell and the external environment, and plays an important role in maintaining the cell microenvironment and normal metabolism. However, plants are often affected by external adverse factors, and the stress tolerance of different ...
- Plant Root Tip Pressing Experiment SummaryThe root tip compression technique can be used to: study chromosome number and morphology in mid-stage plants.Operation methodPlant Root Tip Pressing ExperimentPrincipleMitosis is the main way of plant cell division and proliferation of somatic cells. During mitosis, the chromosomes in ...
- Experiments on the meiotic preparation of plant pollen mother cells SummaryMeiosis is a specialized type of mitosis that occurs in organisms during the maturation of the gametes formed by the gametocytes, and it consists of two consecutive cell divisions, the first of which is meiotic and the second of which is isochronous. The first division has a longer ...
- Segregation-independent assignment of genes and intercropping experiments SummaryMendel's laws include the "law of segregation" and the "law of independent distribution". According to the law of segregation, a pair of alleles located on a pair of homologous chromosomes will segregate from each other during meiosis to form gametes, and will be assigned to different ...
- Experiments on the detection of plant respiration rate by the air flow method SummaryThe principles and techniques of determining the rate of plant respiration by the airflow method will be mastered through experiments.Operation methodExperiments on the detection of plant respiration rate by the air flow methodPrincipleThe principle of the air flow method to determine the ...
- Chromosome number variation experiment Summary1. to understand the principles and methods of artificially induced polyploid plants and their significance;2, to observe the various variations in the number of plant chromosomes and their cytological characteristics during mitosis.Operation methodChromosome number variation ...
- Plant DNA extraction and quantitative analysis experiments SummaryThe cells of young tissues are in the stage of exuberant division, with larger nuclei and less cytoplasm, high concentration of nucleic acid, and fewer inclusions, fewer secondary metabolites, and relatively fewer proteins and polysaccharides. In the presence of SDS or CTAB substances, ...