Experimental methods of Agrobacterium-mediated transformation of barley
Agrobacterium-mediated transformation of barley has been widely utilized over the last decade and it offers advantages over gene gun-mediated transgenic techniq
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Fluorescence in situ hybridization for the detection of transgene fragments integrated in plant genomes experiments
Over the past 30 years, fluorescent chromosome analysis techniques have facilitated the understanding of genome construction and deepened knowledge of the organ
Experiments on the design and management of field trials of transgenic crops
With the development of transgenic technology systems, the transfer of heterologous genes into the plant genome has become possible, which provides a new avenue
Substantial equivalence (proteomics) experiments
Wheat, as one of the major global crops, is processed into a range of food products for consumer consumption. Therefore, it is of great importance to determine
Substantial equivalence (transcriptomics) experiments
In Western Europe, for transgenic crops to be approved for commercial production, proof of their substantial equivalence to conventionally bred crops needs to b
Substantial equivalence (metabolomics) experiments
Modern "metabolomics" methods can be used to automatically compare the levels of a number of structurally different compounds in a large number of samples. The
Xylem and phloem sap protein extraction experiments
It is well known that the xylem and primordial vasculature in higher plants are responsible for the long-distance transport of small nutrient molecules. However
Experimental proteomic analysis of Arabidopsis thaliana chloroplasts
This chapter describes a simple and practical method for large-scale isolation and purification of chloroplasts. The advantages of this method are high yield, l
Cereal plant seed protein extraction experiment
Seeds may contain different components, like starch and polysaccharides, which can severely reduce the amount of protein extracted. The proteins in cereal plant