Experiments for the determination of root vigor by the TTC method
Triphenyltetrazolium chloride (TTC) is a redox substance with a standard redox potential of 80 millivolts. It is a colorless solution when dissolved in water, b
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RNA-mediated gene silencing assay
Down-regulation of endogenous gene expression by post-transcriptional horizontal gene silencing (PTGS) is a key to characterize plant gene function. Many RNA le
Experiments on the induction of α-amylase formation by gibberellin
Gibberellins are produced in the dextrinous layer of the embryo after water absorption and germination of barley or wheat seeds. These gibberellins, when releas
Fat Extraction - Soxhlet's Fat Extraction Method Experiment
In the determination of the oil content of the sample, usually using organic solvents with a boiling point of less than 60 ℃ as a fat solvent (such as ether an
Determination of plant transpiration rate
The speed of transpiration is related to the speed of mineral salt transportation in the plant and leaf temperature, etc. The transpiration rate can be used as
Determination of coolant content in plant tissues
Phenolic substances in plant tissues are oxidized to Da by polyphenol oxidase, and Da can automatically polymerize into colorful substances and browning occurs.
Plant DNA Extraction
The extraction of plant DNA is a basic technique in plant molecular biology and genetic engineering research, and several methods such as CTAB method, SDS metho
Phenylalanine deaminase (PAL)
Phenylalanine ammonia-dissolving enzyme (PAD) plays an important role in regulating the formation of secondary substances such as lignin, phytoalexins, flavonoi
Seed assay by triphenyltetrazolium chloride (TTC) method
Viable seed embryos undergo continuous redox reactions during respiration, and the hydrogen shed reduces the coenzymes (NAD or NADP). When TTC penetrates into t