Laboratory Protocols & SOPs Library
- Protein identification using nanoscale liquid chromatography-tandem mass spectrometry SummaryTandem mass spectrometry is an effective technique for peptide characterization based on the fragmentation pattern of the peptide, also known as MS/MS scanning. In tandem mass spectrometry, each peptide fragment can obtain its own spectrum, which allows the identification of proteins ...
- Nanoscale two-dimensional liquid chromatography tandem mass spectrometry of rice leaf and root proteins SummaryIn this chapter, we present a detailed experimental method for the large-scale identification of proteins in rice leaf and root tissues using 2D liquid chromatography tandem mass spectrometry. The method is realized on a nanoscale flow rate dual-phase column (strong cation ...
- Observational experiments on plant morphological diversity SummarySource: Experiments in BotanyOperation methodbasic programPrincipleThere are hundreds of thousands of species of plants living on Earth, each with its own morphological characteristics. The morphology of the same plant may vary to different extents due to both genetic and environmental ...
- Electroelution of intact proteins from SDS-PAGE gels and their analysis by MALDI-TOF MS SummaryA method has been developed for extracting intact proteins from SDS-PAGE gels and analyzing them by MALDI-TOF MS to determine their molecular mass. This method consists of an electroelution step and a low-temperature matrix/analyte co-crystallization step prior to MALDI-TOF MS analysis. ...
- Seed plant identification experiments SummaryPlant identification is a means of determining the name of a plant, checking the name of a plant rather than fixing a name. Nomenclature is to name a newly discovered species or to verify the correctness of an established name. Source: Experiments in BotanyOperation methodbotanical ...
- Experiments on the construction of plant protein microarrays and the study of antigen-antibody interactions SummaryThe completion of the Arabidopsis and rice genome sequencing projects has greatly supported the application of proteomics methods such as protein microarray technology. In this chapter, we present a method for the construction of plant protein microarrays and its application to the study ...
- Using plant protein microarrays to study protein phosphorylation experiments SummaryIdentification of protein kinase substrates is a major focus of protein kinase research and provides evidence for resolving signal transduction pathways and their complex regulation. We present here an ex vivo experimental method based on protein microarray technology for systematic ...
- Experiments on the observation of anthocyanin and inorganic salt crystallization in plant cells SummarySource: Experiments in BotanyOperation methodObservation of anthocyanin and inorganic salt crystallizationMaterials and InstrumentsOnion bulbs DuckweedBlade. Microscope.Move1. Anthocyanin Take an onion bulb and cut it into four parts with a razor blade. Choose 1 piece of scale leaf, in ...
- Observational experiments on flowering and pollination processes SummarySource: Experiments in BotanyOperation methodbasic programPrincipleSexual mating determines the transmission of genes at the population level and thus has had a profound effect on plant evolution, and there is a wide variety of mating patterns in angiosperms. Pollination within the same ...