Plasmid preparation
Plasmid preparation can be used for (1) carrying exogenous genes into bacteria for amplification or expression of the main vector, which has an important role i
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Gene cloning: efficient receptor cell production
Highly efficient receptor cells can be used for plasmid transformation.DNA cloning refers to the process of molecular manipulation in which a DNA fragment conta
Amplification experiments for stickies and plasmid libraries
The library should be amplified as soon as possible once it is packaged; it can greatly increase the copy number of the library, but there may be some potential
Amplification experiments of phage libraries
The library should be amplified as soon as possible once it is packaged; it can greatly increase the copy number of the library, but there may be some potential
Sieving agarose gel electrophoresis experiments
Sieve agarose gels spread and electrophoresis like regular agarose gels, but they can resolve small fragments of DNA as well as non-denaturing polyacrylamide ge
Ligation, transformation and screening of recombinant plasmids
Recombinant plasmid ligation, transformation and screening are mainly used to obtain recombinants containing the target gene linkage.
Experimental chemiluminescent detection of digoxin-labeled probes
The digoxin system is over provided as another non-isotopic labeling method, which is detected by coupling an anti-digoxin antibody to one or more fluorescent d
Detection of methylation by sequencing after bisulfite modification
Bisulfite-modified sequencing can be used primarily to detect methylation.
Stability screening assay for transfected cells
This experiment is mainly used to obtain eukaryotic cells containing the target exogenous gene.