Interphase FISH study experiments in chronic myeloid leukemia
The purpose of this chapter is to apply fluorescence in situ hybridization to the study of interphase nuclei in patients with chronic myeloid leukemia (CML). In
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Hybridization and data processing experiments
By determining the ratio of fluorescence at each immobilized cDNA spot, one can measure the relative amount of messengers in one sample library to another. If a
PCR-based differential subtractive cDNA cloning experiment
Differential cloning is an effective technique for isolating and characterizing mRNAs that are differentially expressed in two cell populations. The cell types
Fluorescence immunophenotyping and FISH co-analysis experiments on tumor cells
Chromosomal aberrations are usually detected in most hematological and various solid tumors and, clinicopathologically, are frequently associated with the deter
Chromogenic in situ hybridization and FISH in pathology
Fluorescence in situ hybridization (FISH) techniques enable rapid detection of chromosomal abnormalities in a variety of tissues, including fresh and archival s
PCR products electrophoretic silver staining assay
There are two commonly used nucleic acid electrophoresis methods. ① Horizontal electrophoresis: agarose gel, bands are judged under UV light. This method is ec
Poxvirus purification assay
Poxvirus is usually purified by sucrose density gradient centrifugation. The purified virus can be used to prepare poxvirus DNA for use in studies that do not a
Differential reduction cDNA library creation experiment
Differential subtraction libraries contain mRNA cDNA clones that are present in one cell or tissue but not in another. This cDNA library is used to isolate a se
FISH assay for detection of HER2 amplification in breast cancer
Gene amplification is frequently detected in human tumor cells and plays an important role in tumorigenesis. Genome-wide scans of tumor cells using comparative