Chromosome GTG specimen preparation experiment
Non-banded chromosomes are difficult to identify except for some chromosomes based on their morphology, whereas the banding technique allows the appearance of d
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M13 Experiments on the preparation of phage-derived vectors
M13 phage is a filamentous phage that does not lyse bacterial cells after infection, but only utilizes intracellular material to complete its own proliferation,
Extraction of plasmid DNA from Escherichia coli (alkaline lysis)
The basic principle of plasmid DNA extraction by alkaline lysis is that solution I suspends the cells and EDTA can chelate with Ca2+ and Mg2+ to inhibit the act
Construction of DNA libraries and their screening
The construction and screening of DNA libraries can be used to: (1) cut the entire genomic DNA of an organism into DNA fragments of a certain length by restrict
Chromosome CBG specimen preparation experiment
Heterochromatin is condensed throughout interphase and metaphase and is therefore relatively constant in size. They are usually located near the mitotic grains
cDNA library construction
The cDNA library construction can be: (1) used to isolate full-length genes for gene function studies; (2) screen target genes and use them directly for express
Formaldehyde denaturation electrophoresis of RNA
Formaldehyde denaturation electrophoresis of RNA can be used for: (1) after extracting the total RNA of the sample, the quality of RNA is generally judged accor
Bacterial Genome DNA Extraction
Bacterial genomic DNA extraction can be applied to: (1) obtain bacterial genomic DNA; (2) as PCR template; (3) for sequencing, genetic information analysis, etc
Labeling experiments with DNA probes
Nucleic acid probe molecular hybridization refers to the two nucleic acid single strands with certain homology can be formed under certain conditions according