3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
DNA fragments recovered from agarose gels, such as PCR products or digested DNA fragments, are resistant to further digestion. The reason for this resistance is
This protocol is a modified version of the method described by Kupiec et al. (1987), which consists of digestion of cells and tissues with proteinase K, separat
This protocol, modified from the method of Ramirez-solis et al. 1992, 1993, is a simple and efficient method for extracting genomic DNA from eukaryotic cells gr
This method is a modification of the Bowtell method (1987), which allows for the simultaneous preparation of DNA from many different cell or tissue samples.The
Yeast DNA prepared according to this protocol can be used as a template for PCR reactions. The shuttle plasmid, which replicates in both E. coli and Saccharomyc
DNA prepared by this method is suitable for agarose gel electrophoresis, Southern blotting, subcloning, genomic library construction, PCR, or other methods that
This simple protocol is widely used in hundreds of laboratories for genotyping transgenic or knockout mice, and for extracting DNA from small amounts of culture
Genomic DNA samples are prepared by digestion with one or more restriction endonucleases, and the digested fragments are separated by electrophoresis on a stand
The dot and narrow-line hybridization technique (Kafatos et al. 1979) is used to immobilize several nucleic acid samples on the same solid-phase support (usuall
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