Isolation of RNA according to size: agarose gel electrophoresis of glyoxalized RNA
This method combines glyoxal denaturation and agarose gel electrophoresis (modified from that of McMaster and Carmichael (1977), Thomas (1983)). automated glyox
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Isolation of high molecular mass DNA from mammalian cells using proteinase K and phenol
This procedure is adapted from a method first described by Daryl Stafford and colleagues (Blin and Stanfford 1976). This method should be used when large quanti
Recovery of Concentrated DNA Fragments from Pulsed Field Gels
DNA from low melting point agarose sections is first concentrated by electrophoresis into high percentage agarose gels and then separated by treatment with agar
Northern hybridization
RNA samples transferred and immobilized to membranes can be hybridized to specific probes that can be used to localize the RNA of interest. Depending on the exp
Transfer and immobilization of denatured RNA on membranes
In most cases, to detect a specific target mRNA, the RNA is separated by agarose electrophoresis and then transferred from the gel to a two-dimensional support,
Simultaneous Preparation of DNA, RNA and Proteins from Cells and Tissues in a One-Step Process
Like the rapid RNA extraction method reported by Chomczynski and Sacchi in 1987, this method consists of lysing cells with a single-phase solution containing gu
Detection of DNA staining in polyacrylamide gels
Unlike agarose gels, ethidium bromide cannot be added to polyacrylamide gels as this dye interferes with the polymerization of acrylamide. However, ethidium bro
RNA mapping with S1 nuclease
Three different nucleases, S1 nuclease, RNAase, and exonuclease VII, are used to quantify RNA, to identify intron positions, and to characterize the positions o
Flat-end cloning of PCR products
After the target gene is amplified by PCR and the sample is purified and recovered as necessary, the next step is to use flat ends for ligation and cloning, whi