Isolation and purification of T cell subsets
Isolated T cells are categorized into CD4+ and CD8+ T cells using anti-CD8 antibody. Since both types of cells can be recovered, this method is superior to anti
Knowledge Base
Laboratory Protocols & SOPs Library
3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
3228 articles
Activation of mouse macrophages
Macrophage activation goes through two phases: a sensitization phase and an activation phase. While most protocols for macrophage activation use a one-step appr
Isolation of human intestinal mucosal single nucleated cells
Isolation of single nucleated cells from human intestinal mucosa Author: J.E. Colligan et al, Translated by Xuitao Cao et al. This experiment is from the "Compe
Isolation of single nucleated cells from peripheral and umbilical cord blood
Peripheral blood mononuclear cells can be used in: (1) immunology (including autoimmune diseases), infectious diseases, hematologic malignancies, vaccine develo
Detection of T-cell killing function
The assay of T-cell killing function can be applied to (1) immune cell function determination (2) clinical immunodiagnosis.
Monoclonal antibody ascites preparation experiment
The primary advantage of using monoclonal antibodies (MAb) over polyclonal antisera is the possibility of obtaining large quantities of specific monoclonal anti
Cloning and Expansion of Human T Cells
Induction of antigen-specific T cells Author: J.E. Colligan et al, Translated by Xuitao Cao et al. This experiment is from "Comprehensive Immunology Laboratory
Immune molecules and receptor engineering
A method for the preparation of soluble M H C -I g dimers is described, and a method for the preparation of multimeric M H C -I/peptide complexes is presented.
Experiments on the preparation of antigens and immune sera
Injecting the antigen into the animal body can stimulate the animal's B lymphocytes to transform into plasma cells and produce specific antibodies. When a large