Preparation of polyclonal antibodies against phosphopeptides.
It is possible to prepare anti-phosphopeptide antibodies (i.e., antibodies that recognize phosphorylated peptides) that recognize only proteins in the phosphory
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Experiments on cyclic precipitation reactions
The cyclic precipitation reaction is widely used in forensic medicine for the identification of bloodstains and the determination of food adulteration. Usually
IHC sensitization test
There are several ways to increase the sensitivity of IHC. One is to rely on various IHC pre-treatments, such as enzyme digestion and antigen repair at high tem
Electron microscopy enzyme cytochemistry experiment
Currently there are three main classes of enzymes that can be localized by electron microscopy; they are hydrolytic enzymes, oxidative enzymes, and transferase
mast cell staining assay
Mast cells are derived from undifferentiated mesenchymal cells and are found in tissues such as perivascular submucosa, loose connective tissues, tumor tissues,
two-way immunodiffusion assay (BIDA)
The diffusion of antigen and antibody in a gel and the precipitation reaction is called immunodiffusion. An antigen and its corresponding antibody are placed in
Calcium staining assay
Calcium is deposited in bone tissue or pathologically altered tissue, most often in calcified tissue such as tissue necrosis, atherosclerosis and tumors. In the
Perfusion fixation experiments
Source: Practical Experimental Techniques in Neurobiology, Fourth Military Medical University Press
T and B lymphocyte separation assay
Lymphocytes are divided into two main subpopulations, T and B lymphocytes, which have different properties and functions and are used for (1) immunological stud