Isolation of suspension cell clones
Aspirate colonies with a spiking gun or Pasteur pipette and transfer to culture wells in culture flasks or multiwell plates.
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Culture of cerebellar granule cells
Cerebellums from 4-8 neonatal rats were cut into small cubes, placed in HBSS, and digested with trypsin for 15 min at 37°C. Cells were inoculated into petri
Separation of cell colonies by radiation
Place the culture bottles upside down under an X-ray machine or 60Co source and shade the desired colonies with a lead block.
Cloning culture in Mexisol
Cells were suspended in medium containing Mexisol, and such cells were seeded in Petri dishes lined with an agar (or agarose) gel base.
Separation of cell clones by cloning rings
Colonies were digested with trypsin in porcelain cloning rings, glass rings, PTFE rings, or stainless steel rings and transferred to one well of a 24-well or 12
Experiments on the isolation and culture of colonic crypts
Although there have been many reports of continuous cell lines for human colon cancer, there are fewer reports of normal intestinal epithelial cultures.Owens et
Olfactory bulb sheath-forming cells
This lab was derived from: Animal Cell Culture - A Guide to Basic Techniques (5th Edition)
Clonal culture in agar
The agar is liquid at high temperatures, but becomes a gel at 37°C. Cells are suspended in warm agar gel medium and cultured after the agar has formed a gel, f
Mammary epithelial cell culture experiment
Breast milk and mastopexy excised tissue are suitable sources of material for normal mammary ductal epithelium, and purer epithelial cells can be obtained from