DNA assay with Hoechst 33258
Homogenize cells or tissues in buffer, followed by sonication. An amount of the cell suspension is mixed with Hoechst 33258 and the fluorescence is determined.
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Crystalline violet staining
This lab was derived from: Animal Cell Culture - A Guide to Basic Techniques (5th Edition)
Chromosome Preparation
Cells blocked in mid-division were fixed, swollen in hypotonic solution, dropped on slides, stained, and observed [ Rothfels and Siminovitch, 1958; Rooney and C
Giemsa stain
Cultures were fixed with methanol and stained directly with undiluted Giemsa's solution, then the stain was diluted 1:10, the cultures were washed, and observed
Cell counting experiments with blood cell counting plates
Digest the monolayer culture with trypsin, or take a sample from the suspension culture; prepare a coverslip and add the cells to the small chamber of the hemoc
Experiments for plotting growth curves of cells cultured in monolayers in culture flasks
Following passaging culture, cells assume growth forms characterized by delayed, exponential or logarithmic, and stationary (plateau) phases. The logarithmic an
Experiments for drawing growth curves of cells cultured in monolayers in multiwell culture plates
Three sets of cells were cultured in multiwell plates at three different cell concentrations, and cells in one plate were counted each day before reaching plate
Preparation of media with dry powder
Dissolve all the dry powder in the package with appropriate amount of ultrapure water: put the container on the magnetic stirrer and add the dry powder while st
Preparation of special media
Prepare storage concentrates and freeze for preservation. Thaw, mix, aseptically filter and store when needed.