Polylysine copolymers for gene delivery
Polylysine and its copolymers have gained wide application as non-viral gene vectors. Although polylysine itself is toxic to cells, the covalent attachment of p
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Labeling experiments with fluorescence in situ hybridization probes for genomic target sequences
Fluorescence in situ hybridization (FISH) requires nucleic acid probes, including deoxyribonucleic acid (DNA), ribonucleic acid (RNA), or nucleic acid analogs t
Gene gun technology for delivering genes into the skin
Gene gun technology is a fast and simple technique for delivering genes into cells. This technology has many advantages: it allows direct delivery of plasmids w
Cyclodextrin-containing polycations for nucleic acid delivery
A large number of non-viral systems have been used for nucleic acid delivery to cultured cells in vitro or to specific cells in vivo. They vary considerably in
Experiments on the isolation of subcellular organelles and subcellular structures
In proteomics research, a key step in all enrichment techniques is the subcellular fractionation, which is particularly important for the analysis of intracellu
Experiments on mifepristone-induced gene regulatory systems
The mifepristone-induced gene regulatory system (M I G R S ) is a system for regulating rake gene expression through the antiprogesterone drug, mifepristone (M
Non-viral gene transfer experiments with Trojan horse liposomes that can cross the blood-brain barrier
Non-viral vector plasmid DNA can be delivered to the human brain via the vascular route across the blood-brain barrier by intravenous injection of DNA encapsula
Experiments with biodegradable nanoparticles
Biodegradable nanoparticles are colloidal particles, typically around IOOnm in diameter, which can be prepared from biodegradable polymers such as polylactic ac
Site-specific integration experiments mediated by bacteriophage ΦC31 integrase
Efficient delivery of ΦC31 integrase and donor plasmids to recipient tissues or cells of interest remains the most challenging element of this integrative syst