Sequencing Principles and Procedures for the ABI Model 310 DNA Sequencer
DNA sequencing is divided into manual sequencing and automated sequencing, and manual sequencing includes the Sanger double deoxy chain termination method and t
Knowledge Base
Laboratory Protocols & SOPs Library
3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
3228 articles
Liquid Nitrogen Grinding
Liquid Nitrogen Milling for Applications Related to Deoxyribonucleic Acid (DNA) Isolation
Apparent molecular weight determination experiment
Source: Laboratory Guide to Proteins and Proteomics
Source and culture experiments of human embryonal carcinoma (EC) stem cell lines
Teratocarcinomas are structurally diverse tumors that typically consist of a series of differentiated cell types that form a teratoma and a population of stem c
Experiments in the culture of human germ cell lineages
This chapter focuses on embryonic germ cells (E G C ) and aims to provide a series of experimental protocols that can be utilized to understand the relationship
IMViC and Hydrogen Sulfide Experiment
IMVIC is an acronym for four tests: indol test, methyl red test, Voges-Prokauer test and citrate test. i is added in English for ease of pronunciation. These fo
Feulgen staining of DNA
DNA is hydrolyzed by a weak acid (1 mol/L HCl), and the bonds between the purine bases on it and the deoxyribose sugar are opened, causing the formation of free
PCR-ELISA Telomerase Assay
Telomeres are specific DNA-protein structures at the ends of eukaryotic chromosomes, and telomeric DNA is a series of repetitive G-rich DNA sequences, which are
Basic techniques for the preparation and use of PPA
Cross-linking of the initially purified SPA with HRP by sodium periodate oxidation (cross-linking reaction time at 22°C for 5h) gives PPA.