indirect immunofluorescence
The cells were fixed and primary and secondary antibodies were added sequentially and visualized by UV light.
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Protein synthesis experiments
The colorimetric method can determine the amount of total protein at any point in time. Continuous observation over a period of time can be used to determine ne
Experiment on the determination of protein content by Bradford's method
Dissolve proteins, mix with dye, and read O.D. after 10 min. Source: Animal Cell Culture: A Guide to Basic Techniques, Fifth Edition
Isozyme analysis
Cells were collected, washed with D-PBSA and reconstituted into a cell suspension of 5×107 cells/ml, cell extracts were prepared and stored at -70°C.
Clean Incubator
Move the culture to another incubator, turn off the power to the empty incubator, then scrub it with detergent and ethanol and turn on the heat switch in order
Filtration with syringe-type filtration for bacteria transport
This lab was derived from: Animal Cell Culture - A Guide to Basic Techniques (5th Edition)
Vacuum filter bottle sterilization and filtration experiment
This lab was derived from: Animal Cell Culture - A Guide to Basic Techniques (5th Edition)
Fluorescence detection of Mycoplasma
Cells were cultured for at least one week in the absence of antibiotics, culture supernatants were transferred to the indicated cells in early logarithmic growt
Experiments for plotting growth curves of suspension-cultured cells
A series of cell cultures was performed at three different cell concentrations and cells were counted daily until plateau. Source: Animal Cell Culture: A Guide