Polyacrylamide gel electrophoresis of serum proteins
Serum protein polyacrylamide gel has the advantages of good mechanical strength, elasticity, transparency, high chemical stability, non-electroosmosis, simple e
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Experimental extraction of calmodulin from chicken gizzard
Calmodulin is found in all mammalian tissues and also in other eukaryotic organisms. It is one of the most ubiquitous and highly conserved proteins known. This
Phenyl-Sepharose chromatographic assay of calmodulin
This protocol has been developed over many years of practice, and it is not an example of a first attempt to purify a protein by HIC chromatography. For calmodu
DEAE SEPHADEX A-50 anion-exchange chromatography assay for calmodulin
The next step in the purification of calmodulin is anion exchange chromatography with DEAE resin. This step takes advantage of the fact that calmodulin is a str
Protein sequence analysis and structure prediction experiments
Source of content: Guangdong Pharmaceutical University Laboratory Instruction Manual.
Titration curve experiment
The titration curve is a curve presenting the mobility of each component of the sample against pH, for which the gel containing the carrier amphoteric electroly
Urea gradient polyacrylamide gel electrophoresis and folding analysis experiments
Goldenberg and Creighfn (1984) introduced the use of polyacrylamide gels containing a concentration gradient of urea to analyze the folded state of proteins. A
Crude graded separation experiments of calmodulin
For the purification of calmodulin, two different operations were performed for the crude graded separation of smooth muscle tissue extracts: ammonium sulfate p
Activation assay of calmodulin samples
This experiment describes the process of calreticulin sample activation measurement. This experiment is from the Experimental Guide for Protein Purification and