Gel mobility change analysis experiment
In this experiment, we used the same complementary oligonucleotide used for DNA affinity chromatography (see the end of the Introduction to Experiment 3 for the
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How to load gel filtration column experiment?
This experiment describes how to load a gel filtration column. This experiment is from Protein Purification and Identification Laboratory Guide by Houzhu Zhu.
Sephacryl S-300 HR column chromatography of HeLa cell nucleus extracts
This experiment describes the process of Sephacryl S-300 HR column chromatography of HeLa cell nucleus extract. This experiment is from the guide to protein pur
Quantitative protein spot blotting assay
Protein blotting, which uses immunological methods to measure the amount of a particular protein. This experiment was derived from Protein Purification and Iden
Experiments on purification of oligonucleotides by preparative gel electrophoresis
This experiment describes how to purify oligonucleotides by preparative gel electrophoresis. This experiment is from Protein Purification and Identification Lab
Heparin-Sepharose CL-2B preparation experiment
This experiment describes the preparation of heparin-Sepharose CL-2B. This experiment was derived from the Experimental Guide for Protein Purification and Ident
Experiments for the determination of the number of insulin receptors on membranes
Insulin binds to its receptor with high affinity (KD~109 mol/L), and this ability allows us to estimate the number of insulin receptors in even fairly crude pre
Insulin receptor solubilization and activity assay experiments
This experiment describes about the process of solubilization and activity determination of insulin receptor. This experiment is from the guide to protein purif
Precipitation σ32 and RNA polymerase assays
This experiment describes the amount of polyethyleneimine needed to precipitate σ32 and RNA polymerase. This experiment was derived from Protein Purification a