Site-specific nucleic acid endonucleases for protein engineering experiments
Site-specific nucleic acid endonucleases are involved in many aspects of nucleic acid biochemistry. Restriction enzymes and related enzymes have become the para
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3228 articles
DNA fragmentation and directed evolution experiments using nucleotide exchange and shearing techniques
The use of DNA mashups to mimic natural evolutionary processes is a common approach to optimize DNA and protein properties. Here we present a new development in
Experiments on combinatorial protein design strategies using computational methods
Computational methods have always played an important role in protein design. This work focuses on searching the protein sequence space to find one or several p
Calcium indicator experiments based on the fusion of calmodulin and fluorescent protein
Calmodulin (CaM) is a ubiquitously distributed protein involved in calcium-involved signaling. Upon Ca2+ influx, CaM acquires a strong affinity for binding vari
Simplified oligonucleotide gene mixing assay
Improvements in the biochemical properties of proteases can be achieved through misdirected mutagenesis and DNA hybridization of the enzyme genes. The hybridiza
Antibody simulation experiments based on the framework of fibronectin type III structural domains
We developed the use of the 10th fibronectin type III structural domain (FNfnlO) of human fibronectin as a framework to show the use of multiple surface chain s
Integral adulteration experiments of non-natural amino acids in Escherichia coli
The doping of amino acid analogs is becoming increasingly useful. The targeted incorporation of non-natural amino acids has made it possible to use chemical bio
Experiments in compartmentalized self-replication (a new approach to the directed evolution of polymerases and other enzymes)
Compartmentalized self-replication (CSR) is a novel approach to the directed evolution of proteases, especially polymerases. In the simplest form of CSR, it con
Synthesis of a parsimonious evolutionary library of the Ras-binding structural domain of the Raf protein and cloning experiments for rapid screening of dihydrofolate reductase using the fragment complementation method
The field of protein engineering is primarily concerned with the most fundamental sequence determinants regarding the design of new enzyme activities or folds,