Screening experiments of descaling agents for solubilizing membrane proteins
Although TriumX-100 is a good descaling agent for solubilizing insulin receptors from murine liver plasma membranes, it may not be suitable for solubilization o
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Separation of phosphopeptides with iminodiacetic acid (iron chelate) Sepharose 6B
In the structural and functional analysis of proteins (phosphorylated proteins), it is useful to be able to separate phosphopeptides from unmodified (unphosphor
SDS-PAGE protein sample preparation
This experiment describes the preparation of SDS-PAGE protein samples.
silver dyeing experiments
This experiment describes the process of gel staining silver staining. This experiment is from the Protein Purification and Identification Laboratory Guide by Z
Insulin affinity chromatography assay
In this experiment, we used insulin agarose column chromatography as the final step in insulin receptor purification. This method utilizes the high affinity bin
Lectin affinity chromatography experiments on solubilized receptors
This experiment describes the process of lectin affinity chromatography on solubilized receptors. This experiment was derived from Protein Purification and Iden
Performic acid oxidation experiments on proteins
Oxidation of proteins with peroxyformic acid is a simple and effective method of partially denaturing proteins and stabilizing sulfur-containing amino acids. Pe
Analytical experiments on glycosylation of insulin receptors
This experiment describes about the analysis of insulin receptor glycosylation. This experiment is from the Protein Purification and Identification Lab Guide by
Precipitation experiment with trichloroacetic acid
TCA precipitation experiments are mainly used for (1) precipitation purification of proteins and (2) extraction of specimen compounds.