3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
More information about phosphopeptides can be obtained by digestion with sequence-specific proteases or cleavage with site-specific chemicals. From The Compact
CE is particularly useful for separating specific peptides from complex mixtures such as proteolytic products. The following protocol describes the use of the P
Although many different precipitation methods have been used over the past 100 years, AS precipitation has been the most widely used, especially for acidic prot
No method can directly quantify the purity of a protein sample. Typically, protein purity determination involves evaluating the level of specific impurities or
SDS-PAGE has proven to be an extremely useful analytical method in determining the number and size of peptides in a sample. It also has the ability to separate
This chapter describes electrophoretic-based protein assays, focusing on the current methods for total protein assay, post-translational modification (PTM) assa
Membrane proteins play a key role in biological processes. They are involved in cell-cell and cell-matrix interactions, cell organelle and cell formation, trans
Hydroxyapatite (HA) is a calcium phosphate-based hydroxylate that has been used extensively in the chromatographic separation of proteins, mainly in 1991-2009,
Protein kinases are analyzed using labeled donor substrates, and the accumulation of markers in protein or peptide acceptor substrates is readily detected when
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