Chemical degradation of fusion proteins
The use of gene fusion expression systems to express exogenous genes in E. coli has become increasingly popular. The reason for this is largely attributed to th
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Discontinuous nondenaturing coagulation limb electrophoresis experiments
Non-denaturing gel electrophoresis, also known as natural gel electrophoresis. The separation of a protein in a gel depends on its charge and molecular size. Wh
Solubilization and replication experiments of inclusion body proteins
Although inclusion bodies are mixtures of unnatural forms of expressed proteins, the peptide chain itself is intact, or the primary structure is correct. One ad
Batch Chromatography for Protein Concentration
Batch chromatography is simple and fast, suitable for situations where protein resolution is not required, and an effective means of concentrating proteins. Sou
Experimental determination of protein concentration by spotted filter membrane binding method
The spotted filter binding method is a rapid method for the initial determination of protein content. This method is particularly useful for screening useful fr
Capillary electrophoretic analysis of proteins
The main features of capillary electrophoresis are high column efficiency (N＞105-106), short analysis time, low consumption of sample volume and reagents, mult
Preparation of affinity columns
This section will focus on the solid-phase technique of ligand solidification on agarose gels activated with cyanogen bromide. The vast majority of ligands, suc
Tyrosine kinase analysis assay
Protein kinases are analyzed using labeled donor substrates, and the accumulation of markers in protein or peptide acceptor substrates is readily detected when
Determination of protein concentration by Lowry's assay
The Lowry method is a standardized, rapid method for protein quantification and has been widely used to remove interfering substances by protein precipitation p