Dual luciferase reporter assay
The target gene transcriptional regulator original is constructed onto the firefly luciferase reporter gene plasmid, and co-transfected with the sea kidney luci
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Laboratory Protocols & SOPs Library
3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
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Activation and monitoring of inflammatory vesicles
Nucleotide-binding oligomerization domain-like receptor family, pyrin domain-containing 3 (NLRP3) inflammatory vesicles have been well studied in recent years.
Protein Oligomerization Assay
Oligomerization refers to the formation of dimers, trimers, tetramers or long chain molecules (oligomers) between monomeric compounds. In a biological context,
Lipid metabolite analysis
Lipid metabolism is an important source of energy supply for the body, and an imbalance between lipid synthesis and catabolism triggers lipid metabolism disorde
Protein expression system construction
E. coli protein expression is a commonly used protein expression system. E. coli is a common bacterium, which has been widely used in the field of bioengineerin
Yeast two-hybrid technology
Yeast two-hybrid (yeast two-hybrid) is an experimental system for studying the effects of interactions between trans-acting factors on the transcriptional regul
Electrophoretic separation of proteins
Proteins are amphoteric electrolytes and exhibit charged properties in solutions above or below the isoelectric point. These charged proteins migrate by swimmin
Analysis of amino acid metabolites
Amino acids are mainly used in the body to synthesize proteins, peptides and other nitrogenous substances that are unique to the body; on the other hand, they c
Eukaryotic expression of proteins
A protein expression system is a system consisting of a host, an exogenous gene, a vector, and auxiliary components. The vector contains an exogenous gene fragm