PEI Nanoparticles for Targeted Gene Delivery
P E I Due to its polycationic nature, it and negatively charged D N A molecules form nanoparticles through electrostatic interactions, which further help D N A
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3228 articles
HVJ Liposomes and HVJ Packaging Vectors
This chapter describes the construction of a fusion-mediated vector based on inactivated H V J (hemagglutinating virus of Japan, also known as Sendaivirus). The
Solid lipid nanoparticles for in vitro mammalian cell transfection
Solid lipid nanoparticles (S L N ) have many technical advantages over gene carriers such as cationic liposomes and cationic polymers. However, in the absence o
Targeted electrically neutral lipid vesicles for systemic delivery of genes
This chapter describes the detailed experimental procedure for encapsulation of nucleic acids by nanolipid particles (N L P ). This N L P carries a polyethylene
PEGylated nanoparticles of poly(L-lysine) and DNA
Nanoparticles of P E G-coated poly(L-lysine) and D N A are water-soluble, stable in saline and tissue fluids, transfect undivided cells (Liu et al. 2003), show
Transposon-mediated delivery of small interfering RNAs: the "Sleeping Beauty" transposon experiment
A method for efficient delivery of pol III-driven SHRNA expression frames using the Sleeping Beauty (SB) transposon. Advantages of this system include its ease
High-throughput method for screening polymer transfection reagents
The development of a safe and effective gene delivery system is a prerequisite for the success of clinical gene therapy. Despite the high transfer efficiency of
Vesicular stomatitis virus G protein binding assay
The capsid fusion G protein of vesicular stomatitis virus (VSV-G) can be used to prepare pseudotyped retroviruses and pseudolentiviruses, and this glycoprotein
Experiments on nanogene vectors based on polylactic acid and polyethylene glycol
Nanoparticles combining polylactic acid-hydroxyglycolic acid (PLGA) and polyethylene glycol (PEG) derivatives have been used as gene carriers for mucosal permea