Translocation of target genes with surface-modified lentiviral vectors
Two types of surface modifications have been made to lentiviral vectors in order to achieve target gene translocation: (i) taking advantage of the targeting pro
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Generation of VSV-G-pseudotyped retroviral vectors
In order to improve the efficiency of transduction, a strategy called pseudotyping, which combines retroviral particles with other virally encoded capsid protei
Generation of foamy virus vectors and transduction of hematopoietic stem cells
Foamy virus (F V ), or spumavirus, is a nonpathogenic retrovirus developed as an integrating viral vector. Several potential advantages of F V vectors include a
Gene transfer into mammalian cells using targeted filamentous phages
Because of their genetic simplicity, phages are commonly used in vector genetic optimization approaches for therapeutic gene transfer. In addition, since phage
Intrauterine microelectroporation for efficient gene transfer in mouse embryos
The introduction of genes into the embryo has been successfully achieved by a new technique of electroporation of the chicken embryo. In contrast, since the mam
Transfection of hippocampal neuronal cells with calcium phosphate co-precipitated plasmid DNA
The use of DNA/phosphorothioate (CAPO 4) co-precipitation to transfect cells is a widely described method. Although this co-precipitation method was proposed mo
Optimizing in vitro mammalian cell electrotransfection
Electrotransfection can be used for studies of gene function, promoter activity, and gene regulation; it can also be used to create transgenic or knockout embry
HVJ Liposomes and HVJ Packaging Vectors
This chapter describes the construction of a fusion-mediated vector based on inactivated H V J (hemagglutinating virus of Japan, also known as Sendaivirus). The
PEI Nanoparticles for Targeted Gene Delivery
P E I Due to its polycationic nature, it and negatively charged D N A molecules form nanoparticles through electrostatic interactions, which further help D N A