Topic

In situ hybridization

Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.

3 resources · protocols, FAQs & articles
article

Histological Staining Methods for Identifying Hepatitis B Virus: Comparison of HE, Orcein, Aldehyde Fuchsin, Victoria Blue, Immunohistochemistry, and In Situ Hybridization

Identification of hepatitis B virus (HBV) in liver tissue should not rely on a single staining result. HE staining can indicate the pathological background of chronic hepatitis, interface hepatitis, hepatocyte degeneration, fibrosis, and “ground-glass hepatocytes,” but it cannot directly ...

Updated Jul 202612 min read✓ Reviewed
article

Practical Guide to TSA Tyramide Signal Amplification: How to Select Reagents, Optimize the Workflow, and Reduce Background When Detection Signals Are Weak for Low-Abundance Targets

TSA, or Tyramide Signal Amplification, is a signal amplification technique based on HRP (Horseradish Peroxidase)-catalyzed reactions. It is commonly used in IHC (Immunohistochemistry), ICC (Immunocytochemistry), ISH (In Situ Hybridization), FISH (Fluorescence In Situ Hybridization), and ...

Updated Jul 202612 min read✓ Reviewed
protocol

In Situ Hybridization (ISH) Protocol

In situ hybridization (ISH) is an experimental technique used to detect and locate specific nucleic acid sequences (DNA or RNA) in cells or tissues. It uses labeled nucleic acid probes to specifically bind to target nucleic acid sequences, thereby observing and analyzing the expression and ...

Updated Dec 202412 min read✓ Reviewed

Related topics

Techniques often explored alongside immunological experiments.

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