Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
The PLSCR family is commonly used to study changes in membrane phospholipid asymmetry, phosphatidylserine (PS) externalization, cell death, platelet procoagulant activation, and inflammatory stress responses. In experiments, PLSCR expression changes, membrane localization changes, PS ...
The inflammatory response is a protective reaction initiated by the body in response to infection, tissue injury, cellular stress, or disruption of internal homeostasis. Its fundamental purpose is not simply to “produce inflammatory factors,” but to complete three sequential processes: ...
The TNF signaling pathway is one of the most central signaling networks in inflammatory responses, cell fate regulation, and tissue homeostasis maintenance. Its complexity is not reflected in the single fact that TNF can induce inflammation, but in that the same ligand stimulus can, under ...
The GSK3 (glycogen synthase kinase 3) pathway is one of the most representative integrative signaling nodes within the cell. It does not belong to the typical linear "ligand-receptor-kinase" cascade, but is better understood as a constitutively poised regulatory hub whose activity is ...
Sphingolipid metabolism is not merely a process of membrane-lipid turnover, but an important signaling layer linking death-receptor activation, mitochondrial injury, inflammasome assembly, and cell-fate determination.
Techniques often explored alongside immunological experiments.
