Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
Loading Controls are antibodies used for standardizing and quantifying the expression levels of proteins. They typically target proteins encoded by "housekeeping genes," which have relatively constant expression levels across different cell types, tissues, and physiological states, and are ...
Polyclonal, monoclonal, and recombinant antibodies are all suitable for western blotting. Polyclonal antibodies are a collection of many monoclonal antibodies that can vary between immunizations and batches. Polyclonal antibodies recognize multiple epitopes of an antigen and are therefore ...
Updated Jul 202412 min read✓ Reviewed
Related topics
Techniques often explored alongside immunological experiments.
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