Articles by tag "Protein extraction"

1. AEBSF Protease Inhibitor: Properties, Uses & Lab Guide
   posted this at 15 oct 2026

   10
   4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride(AEBSF) is a water-soluble, irreversible serine protease inhibitor widely used in cell lysis, protein purification, and proteomics workflows to prevent unwanted proteolytic degradation of target proteins. Chemical ...
2. AEBSF Protease Inhibitor: Lab Guidelines & Usage Protocol
   posted this at 15 oct 2026

   1
   4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride(AEBSF) is a water-soluble, irreversible serine protease inhibitor widely used to protect proteins of interest during cell lysis, tissue homogenization, and protein purification workflows. Chemical Name4-(2-Aminoethyl)benzenesulfonyl ...
3. Plant EV Isolation & Purification Kit for Juicy Plants
   posted this at 15 oct 2026

   0
   Plant extracellular vesicles Isolation and Purification Kit (from Juicy Plants) is a specialized reagent kit designed to enable researchers to efficiently isolate and purify extracellular vesicles from high-moisture, juicy plant tissues with minimal processing steps and high yield. ...
4. Protease & Phosphatase Inhibitor Cocktails: Research Applications and Principles
   posted this at 16 dic 2025

   31
   During cell lysis, tissue homogenization, and protein extraction, endogenous proteases and protein phosphatases are rapidly activated and brought into full contact with target proteins. This leads to protein degradation and the rapid loss of phosphorylation and other post-translational ...
5. Western Blot Protocol
   posted this at 10 jul 2025

   45
   Product Manager: Harrison MichaelProtein blotting (WB) is widely used to analyze the expression of specific proteins in cell or tissue extracts. Western blot experiments are time-consuming, and their success has a significant impact on research progress. To this end, we have carefully developed ...
6. Surfactant-Free Nuclear Protein Extraction Protocol
   posted this at 23 oct 2024

   26
   Product Manager：Harrison Michael​In the process of nuclear protein extraction, the use of surfactants can significantly interfere with protein labeling efficiency. This is because surfactants can alter the conformation of proteins and may interact with the target protein, affecting its ...
7. Methods of Lysate Preparation for Western Blotting
   posted this at 10 sept 2024

   33
   Licia Miller   Product ManagerThis method provides a recipe of lysate and buffer preparation for lysing cells or tissues for western blot analysis. The buffer solution formula is as follows:( 1 ) 1×1% SDS pyrolysis buffer        - 10 1 mM Tris-HCl (pH 8.0)         - 1% SDS ...