When extracting proteins from suspension cells, can I add the product directly to boil and denature them?
When extracting proteins from suspension cells, can I add the product directly to boil and denature them?
posted this at Jun 9, 2025 Updated at Aug 27, 2024
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When extracting proteins from suspension cells, can I add the product directly to boil and denature them?
Yes, it is possible to boil directly with 1× buffer, but it should be noted that the protein sample buffer obtained after this operation cannot be used for BCA concentration measurement.
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