Phosphatase, Alkaline, CAS No.9001-78-9

CAS: 9001-78-9 Cat. No.: P128629 Número EC: 232-631-4 PubChem CID: 18985873

CALIDAD & PUREZA Native, EnzymoPure™, ≥30 units/mg protein (25°C, pH 8.0),from Escherichia coli

Accession #

P00634

Bioactivity

≥30 units/mg protein (25°C, pH 8.0)

Descripción general

Alkaline phosphatase is a broad term associated with non-specific phosphomonoesterases with an alkaline pH optimum.
Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It may be used for protein labeling when high sensitivity is required.Inibibitors: The enzyme is inhibited by chelating agents and inorganic phosphates.

Unit Definitons

E. coli. One Unit hydrolyzes 1µmole of p-nitrophenol phosphate per minute at 25°C, pH 8.0.

Especificaciones

Product Name

Phosphatase, Alkaline, CAS No.9001-78-9

Sinónimos

Alkaline phosphatase | Phosphatase, alkaline | ENTEROSTATIN,HUMAN | alkaline phosphomonoesterase | glycerophosphatase | phosphomonoesterase | Orthophosphoric-monoester phosphohydrolase (alkaline optimum) | alkaline phosphatase | Apase, Escherichia coli |

Grado

Native, EnzymoPure™

Especificaciones y pureza

EnzymoPure™, Nature, ≥30 units/mg protein (25°C, pH 8.0),from Escherichia coli

Mecanismos bioquímicos y fisiológicos

Alkaline phosphatase, from Escherichia coli, is a dimeric, non-glycosylated protein which mainly reside in the periplasmic space. Three known isoforms exist. The enzyme requires zinc, and is activated by magnesium. E. coli akaline phosphatase has a broad

Bioactividad

≥30 units/mg protein (25°C, pH 8.0)

Número de adhesión

P00634

CAS

9001-78-9

Número de la Comisión de Enzimas

I.U.B.: 3.1.3.1

Tipo de molécula

Enzima

Almacenamiento y envío

Concentración

≥30 units/mg protein (25°C, pH 8.0),from Escherichia coli

Condiciones de almacenamiento de almacenamiento

Store at 2-8°C

Enviado en

Wet ice

Definición de unidad

One Unit hydrolyzes 1µmole of p-nitrophenol phosphate per minute at 25°C, pH 8.0.