Pregnenolone - Moligand™, 10mM in DMSO , CAS No.145-13-1

CAS: 145-13-1 Cat. No.: P408873 Molecular Weight: 316.48 EC Number: 205-647-4

GRADE & PURITY Moligand™, 10mM in DMSO

Synonyms

(3β)​-3-​hydroxy-pregn-​5-​en-​20-​one

Storage

Store at -80°C

Shipped In

Dry ice packs + Cold packs

Overview

Information

Pregnenolone is an endogenous steroid hormone, used in the treatment of fatigue, Alzheimer’s disease, trauma and injuries.
In vitro

Pregnenolone induces a large, dose-related increase of both the rate and extent of MAP2-induced tubulin assembly, whereas progesterone, inactive per se, counteracted the stimulatory effect of Pregnenolone. Pregnenolone-increased assembly of microtubules produces a completely normal structure. Pregnenolone preserves microtubule abundance and promotes cell movement during epiboly. Pregnenolone results in dramatic reduction in GR nuclear localization in mouse hippocampal cell line (HT-22). Pregnenolone has neuroprotective effects against both glutamate and amyloid beta protein neuropathology.

In vivo

Pregnenolone sustains its proliferative activity in vivo and stimulates the growth of LNCaP-tumor xenografts in intact male SCID mice as well as in castrated animals. Pregnenolone is shown to activate transcription through the LNCaP androgen receptor (AR), but not the wild-type AR. Pregnenolone results in a significant decrease in the accumulation of astrocytes in the proximity of the wound and in a decreased bromodeoxyuridine incorporation in reactive astrocytes of rats. Pregnenolone administration results in elevations in downstream neurosteroids such as allopregnanolone, a molecule with neuroprotective effects that also increases neurogenesis, decreases apoptosis and inflammation, modulates the hypothalamic-pituitary-adrenal axis, and markedly increases GABA(A) receptor responses. Pregnenolone administration elevates pregnenolone sulfate, a neurosteroid that positively modulates NMDA receptors.
Cell Data

cell lines：

Concentrations：

Incubation Time：

Powder Purity:≥100%

Specifications

Synonyms

(3β)​-3-​hydroxy-pregn-​5-​en-​20-​one

Specifications & Purity

Moligand™, 10mM in DMSO

Biochemical and Physiological Mechanisms

Pregnenolone is an endogenous steroid hormone, used in the treatment of fatigue, Alzheimer’s disease, trauma and injuries.

Storage

Store at -80°C

Shipped In

Dry ice packs + Cold packs

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

Moligand™

Names and Identifiers

• Isomeric SMILES: CC(=O)[C@H]1CC[C@@H]2[C@@]1(CC[C@H]3[C@H]2CC=C4[C@@]3(CC[C@@H](C4)O)C)C
• Molecular Weight: 316.48
• Reaxy-Rn: 1915502
• Reaxys-RN_link_address: https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=1915502&ln=

Associated Targets(Human)

SHBG Tchem Sex hormone-binding globulin (0 Activities)

Discover Target: SHBG

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Chemical and Physical Properties

• Solubility: Solubility (25°C) In vitro DMSO: 10 mg/mL (65.29 mM); Ethanol: -1 mg/mL

References

1. Xiao-San Li, Mao-Xun Yang, Yu-Hao Luo, He-Hui Zhan, Mei-Fang Chen, Yong-Mei Huang, Li Liu, Xue-Mei Yang.  (2022)  Design, Synthesis and Anticancer Activity of Novel Steroidal Derivatives with D-Ring Fused or Substituted N-Heterocyclic Systems.  CHEMISTRY & BIODIVERSITY,  19  (10): (e202200648).  [PMID:36043381] [10.1002/cbdv.202200648]

2. Yaqin Jia, Zhe Wang, Yuyi Feng, Meixian Wang, Lili Jiang, Zhijun Yu, Xiaoguang Shao, Guiyuan He, Yong Liu.  (2022)  Validity of the association between five steroid hormones quantification and female infertility conditions: A new perspective for clinical diagnosis.  STEROIDS,      [PMID:35803387] [10.1016/j.steroids.2022.109086]

3. Di Xu, Mengyun Wu, Xue Li, Mingyu Xia, Dongchun Liu, Yinghui Dai, Qing Yu, Bin Wu, Dong Wang.  (2018)  Cloning, prokaryotic expression and function of the Bufo bufo gargarizans 3β-hydroxysteroid dehydrogenase (3βHSD) gene.  INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,      [PMID:30170058] [10.1016/j.ijbiomac.2018.08.165]

4. Jixiu Deng, Pengshuai Zhang, Han Sun, Wang Rongrong, Binbin Wu, Yuemei Li, Beibei Feng, Haowei Sun, Shuoye Yang.  (2025)  Insight into the solid-liquid equilibrium behavior and apparent thermodynamic analysis of polymorphic pregnenolone (Form Ⅰ) in thirteen neat solvents.  THERMOCHIMICA ACTA,      [PMID:] [10.1016/j.tca.2025.179979]

5. Ruosi Zhang, Mingdong Yao, Haidi Ma, Wenhai Xiao, Ying Wang, Yingjin Yuan.  (2023)  Modular Coculture to Reduce Substrate Competition and Off-Target Intermediates in Androstenedione Biosynthesis.  ACS Synthetic Biology,      [PMID:36857753] [10.1021/acssynbio.2c00590]