Prohibitin Antibody - Anticuerpo primario, specific to Prohibitin, Rabbit IgG

Cat. No.: Ab123227

GRADE & PURITY Carrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA

Application

IF/ICC, IHC, WB

Host species

Conejo

Reactive species

consejo, Humano, Ratón

Isotype

Rabbit IgG

Conjugation

Unconjugated

Descripción general

Rabbit anti Human Prohibitin Antibody, Polyclonal, could be used for WB, IHC and so on.
Application:

WB: 0.01-2 µg/mL
IHC: 5-30 µg/mL

IF/ICC: 2.5 µg/mL

Protein Function:
Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging.

Specifications

Product Name

Prohibitin Antibody - Anticuerpo primario, specific to Prohibitin, Rabbit IgG

Sinónimos

Epididymis luminal protein 215 antibody | Epididymis secretory sperm binding protein Li 54e antibody | HEL 215 antibody | HEL S 54e antibody | PHB antibody | PHB_HUMAN antibody | PHB1 antibody | Prohibitin antibody | PHB | PHB1 | Prohibitin | HEL-215 | HE

Especificaciones y pureza

Carrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA

Especies hospedadoras

Conejo

Especificidad

Prohibitin

Inmunógeno

Recombinant PHB expressed in E.coli (AA 177-272).

Control positivo

WB: HeLa, MCF7, Ramos, A431, HEK293, Hep G2, Jurkat, A549, NIH/3T3 and C6 cell lysates. IHC: Human breast cancer, rectum, thyroid cancer, liver, liver cancer tissues; Mouse cardiac muscle tissue. IF/ICC: HeLa cells.

Conjugación

Unconjugated

Grado

Azide Free, Carrier Free, ExactAb™, High Performance, Validated

Isotipo

Rabbit IgG

SDS-PAGE

150 kDa

Método de purificación

Antigen affinity purified + Protein A purified

Concentración

See COA

Product Line

Productos biológicos

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imágenes

Western Blot: Prohibitin Antibody (Ab123227)

Prohibitin Antibody (Ab123227) - Western Blot
All lanes: Prohibitin Antibody (Ab123227) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 30 kDa
Observed band size: 28 kDa
Exposure time: 14.8 s

IHC: Prohibitin Antibody (Ab123227)

Prohibitin Antibody (Ab123227) - IHC
DAB staining on IHC
Samples: Human Breast cancer Tissue
Primary Ab: 10 µg/mL Prohibitin Antibody (Ab123227)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: Prohibitin Antibody (Ab123227)

Prohibitin Antibody (Ab123227) - IHC
DAB staining on IHC
Samples: Human Rectum Tissue
Primary Ab: 10 µg/mL Prohibitin Antibody (Ab123227)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: Prohibitin Antibody (Ab123227)

Prohibitin Antibody (Ab123227) - IHC
DAB staining on IHC
Samples: Human Thyroid cancer Tissue
Primary Ab: 10 µg/mL Prohibitin Antibody (Ab123227)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: Prohibitin Antibody (Ab123227)

Prohibitin Antibody (Ab123227) - IHC
DAB staining on IHC
Samples: Human Liver Tissue
Primary Ab: 10 µg/mL Prohibitin Antibody (Ab123227)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: Prohibitin Antibody (Ab123227)

Prohibitin Antibody (Ab123227) - IHC
DAB staining on IHC
Samples: Human Liver cancer Tissue
Primary Ab: 10 µg/mL Prohibitin Antibody (Ab123227)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: Prohibitin Antibody (Ab123227)

Prohibitin Antibody (Ab123227) - IHC
DAB staining on IHC
Sample: Mouse Cardiac Muscle Tissue
Primary Ab: 30 µg/mL Prohibitin Antibody (Ab123227)
Control: Used PBS instead of primary antibody
Second Ab: 2 μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IF/ICC: Prohibitin Antibody (Ab123227)

Prohibitin Antibody (Ab123227) - IF/ICC
IF analysis of Prohibitin Antibody (Ab123227) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Prohibitin Antibody (Ab123227) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).

Aplicación

Aplicación

Dilution info

IHC

5-30 µg/mL

WB

0.01-2 µg/mL

IF/ICC

1/200

Objetivos asociados (humanos)

PHB Tbio Prohibitin (0 Activities)

Discover Target: PHB

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mecanismos de acción

Preguntas frecuentes

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Prohibitin Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.