Recombinant E2F6 Antibody - Anticuerpo primario, specific to E2F6, Rabbit IgG

Cat. No.: Ab100799

CALIDAD & PUREZA Recombinant, ExactAb™, Validated, See COA

Application

WB

Host species

Conejo

Reactive species

Humano

Isotype

Rabbit IgG

Conjugation

Unconjugated

Descripción general

Rabbit anti Human E2F6 Antibody, Recombinant, could be used for WB and so on.
Application:
WB: 1/500-1/2000
Protein Function
Inhibitor of E2F-dependent transcription. Binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Has a preference for the 5'-TTTCCCGC-3' E2F recognition site. E2F-6 lacks the transcriptional activation and pocket protein binding domains. Appears to regulate a subset of E2F-dependent genes whose products are required for entry into the cell cycle but not for normal cell cycle progression. May silence expression via the recruitment of a chromatin remodeling complex containing histone H3-K9 methyltransferase activity. Overexpression delays the exit of cells from the S-phase.

Especificaciones

Product Name

Recombinant E2F6 Antibody - Anticuerpo primario, specific to E2F6, Rabbit IgG

Sinónimos

E2F binding site modulating activity protein | E2F transcription factor 6 | E2F transcription factor 6 isoform 1 | EMA | MGC111545 | Transcription factor E2F6 | E2F6_HUMAN | E2F 6 antibody | E2F 6 | E2F binding site modulating activity protein antibody |

Especificaciones y pureza

Recombinant, ExactAb™, Validated, See COA

Especies hospedadoras

Conejo

Especificidad

E2F6

Inmunógeno

A synthetic peptide derived from human E2F6 (AA 100-281)

Control positivo

WB: K562, Jurkat, Hep G2 and HeLa cell lysates.

Conjugación

Unconjugated

Grado

ExactAb™, Recombinant, Validated

Isotipo

Rabbit IgG

Tipo de cadena ligera

kappa

SDS-PAGE

150 kDa

Método de purificación

Protein A purified

Concentración

See COA

Product Line

Productos biológicos

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imágenes

Western Blot: Recombinant E2F6 Antibody (Ab100799)

Recombinant E2F6 Antibody (Ab100799) - Western Blot
All lanes: Recombinant E2F6 Antibody (Ab100799) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 32 kDa
Observed band size: 32 kDa
Exposure time: 100.0 s

Aplicación

Aplicación

Dilution info

WB

1/500-1/2000

Objetivos asociados (humanos)

E2F6 Tbio Transcription factor E2F6 (0 Activities)

Discover Target: E2F6

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Preguntas frecuentes

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant E2F6 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.