Protocol of Western Blotting For High Molecular Weights
High molecular weight western blotting is a modification of the standard western blotting technique specifically designed for the detection and analysis of large molecular weight proteins.
Topic
Biological buffer
Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
10 resources · protocols, FAQs & articles
Competent E. coli Cell Preparation Using the Calcium Chloride Method
Competent E. coli Cell Preparation Using the Calcium Chloride Method
Ethidium Bromide: Beautiful and Dangerous
Ethidium bromide (EtBr) is a classical fluorescent dye widely used in molecular biology research. Its chemical structure consists of a tri-benzimidazole, which can intercalate between the base pairs of DNA or RNA, significantly enhancing its fluorescence signal. Initially discovered as a ...
Subcellular Fractionation Protocols
Subcellular fractionation is a biological experimental technique that allows researchers to separate and purify different components within cells, such as the nucleus, mitochondria, endoplasmic reticulum, etc., and then further study their structure and function. Centrifugation is one of the ...
The Key to Gene Delivery to Cells —In Vitro Cell Transfection Techniques
Cell transfection techniques are the cornerstone of interdisciplinary research in molecular and cellular biology. They allow scientists to introduce foreign nucleic acids into cells to explore gene function, expression, and develop gene therapies or produce recombinant proteins. This article ...
Guidelines For Common Cell Culture Media
Cell culture medium is an indispensable part of cell biology research, which provides essential nutrients and a suitable develope environment for cells. The following is a comprehensive introduction to the detailed components and characteristics of several commonly used cell culture media:
Method and Precautions of SDS-PAGE Gel Preparation
SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) is a commonly used technique to separate proteins based on size. By denaturating the protein and giving it a uniform negative charge, the protein migrates to the positive pole under the application of an electric field and is ...
Native ChIP (N-ChIP) Protocol
Native ChIP (N-ChIP) Protocol
Immunocytochemistry (ICC) protocol
Immunocytochemistry (ICC) protocol
Biological buffer: the unusual amongst the usual
As a common reagent in the pharmaceutical field, biological buffer plays a crucial role in the process of regulating environmental acidity and alkalescence. With the need of epidemic prevention and control, biological buffer as a laboratory routine supplies in the fight against COVID-19 has also ...
Related topics
Techniques often explored alongside immunological experiments.