UBR7 Mouse mAb - Anticuerpo primario, specific to UBR7, Mouse IgG2a

Cat. No.: Ab133358

GRADE & PURITY Carrier Free, Validated, ExactAb™, 1.0 mg/mL

Application

IHC, IP, WB

Host species

Ratón

Reactive species

Humano

Isotype

Mouse IgG2a

Clone Number

K49039_15G1

Conjugation

Unconjugated

Descripción general

Mouse anti Human UBR7 Antibody, Monoclonal (K49039_15G1), could be used for WB, IHC, IP and so on.
Application:
WB: 1/1000
IHC: 1/1000
IP: 1/100
Protein Relevance:
UBR7 is a putative E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific amino-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation.

Specifications

Product Name

UBR7 Mouse mAb - Anticuerpo primario, specific to UBR7, Mouse IgG2a

Sinónimos

5730410I19Rik antibody | AA589405 antibody | AW557761 antibody | C14orf130 antibody | Chromosome 14 open reading frame 130 antibody | EC 6.3.2.-antibody | FLJ10483 antibody | MGC94098 antibody | MGC9518 antibody | N recognin 7antibody | Putative E3 ubiqui

Especificaciones y pureza

Carrier Free, ExactAb™, Validated, 1.0 mg/mL

Especies hospedadoras

Ratón

Especificidad

UBR7

Inmunógeno

Recombinant protein of human UBR7 (AA 141-420).

Control positivo

WB: HeLa, HEK293, MCF7 and Jurkat cell lysates. IHC: Human testis tissue. IP: T-47D lysates.

Conjugación

Unconjugated

Grado

Carrier Free, ExactAb™, Validated

Isotipo

Mouse IgG2a

Tipo de cadena ligera

kappa

SDS-PAGE

150 kDa

Método de purificación

Protein A purified

Concentración

1.0 mg/mL

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at -80°C,Avoid repeated freezing and thawing

Enviado en

Dry ice packs + Cold packs

Estabilidad y almacenamiento

Store at 2-8 ℃ (3 months). Store at -20 ℃ (12 months). Store at -80 ℃ (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imágenes

Western Blot: UBR7 Mouse mAb (Ab133358)

UBR7 Mouse mAb (Ab133358) - Western Blot
All lanes: UBR7 Mouse mAb (Ab133358) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 48 kDa
Observed band size: 52 kDa
Exposure time: 47.8 s

IP: UBR7 Mouse mAb (Ab133358)

UBR7 Mouse mAb (Ab133358) - IP
Immunoprecipitation was performed by incubation of 2.5 µg of UBR7 Mouse mAb (Ab133358) with T-47D lysate containing 200 µg of total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. UBR7 Mouse mAb (Ab133358) at 1 µg/mL was used as the primary antibody and peroxidase conjugated rabbit anti-mouse IgG (Light chain specific) was used as the secondary antibody. Lane 1: T-47D lysateLane 2: UBR7 immunoprecipitated from T-47D lysate by UBR7 Mouse mAb (Ab133358)
Lane 3: The isotype control antibody instead of UBR7 Mouse mAb (Ab133358) in T-47D lysate.Result: UBR7 Mouse mAb (Ab133358) can immunoprecipitate UBR7. 

IHC: UBR7 Mouse mAb (Ab133358)

UBR7 Mouse mAb (Ab133358) - IHC
IHC analysis of testis tissue by UBR7 Mouse mAb (Ab133358).IHC was performed using sections of the formalin-fixed paraffin-embedded testis tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9.0 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with UBR7 Mouse mAb (Ab133358) at 1 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.Result: Cells in seminiferous ducts are positively stained at the nuclei. 

Aplicación

• Aplicación: Dilution info
• WB:

  1/1000 ;

• IP:

  1/100;

• IHC:

  1/1000

Objetivos asociados (humanos)

UBR7 Tbio Putative E3 ubiquitin-protein ligase UBR7 (0 Activities)

Discover Target: UBR7

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Preguntas frecuentes

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?80 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships frozen with dry ice and cold packs. Unpack on arrival and transfer it to the storage condition stated above; handle dry ice with insulated gloves in a ventilated area.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (UBR7 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.