GRADE & PURITYBioReagent?BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility.≥98%
Storage
Protected from light,Store at -20°C,Desiccated,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Application
Apoptosis, Cell signaling, Drug screening, Enzyme activity assay
BioReagent, ≥98% BioReagent for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Protected from light,Store at -20°C,Desiccated,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Z-DEVD-AMC is a classic Caspase-3/7 substrate, used to detect the enzymatic activity of Caspase-3/7 in vitro or in cell lysis systems. This molecule consists of a tetrapeptide sequence DEVD (a specific recognition sequence for Caspase-3/7) and a fluorophore AMC (7-amino-4-methylcoumarin). Under normal circumstances, AMC is blocked by the DEVD sequence and does not emit light; when Caspase-3 or -7 is activated, it will recognize and cleave the bond between DEVD and AMC, releasing free AMC molecules, which then emit detectable blue fluorescence (Ex/Em ≈ 350/450 nm). Z-DEVD-AMC is often used in microplate fluorescent enzyme activity assays, studies on drug-induced apoptosis, high-throughput drug screening systems, etc., and is particularly suitable for Caspase activity curve analysis and time-dependent experiments. Its advantages include fast reaction speed, strong signal, and low background. However, due to its relatively short excitation wavelength (in the UV region), it is recommended to use a fluorescence microplate reader or fluorescence spectrophotometer equipped with an ultraviolet excitation channel. In addition, it can also be used for fluorescent quantitative detection after cell lysis, but is not suitable for direct imaging of living cells. During operation, attention should be paid to avoiding strong light exposure to reduce the self-luminescence attenuation of AMC.
Specifications
Specifications & Purity
BioReagent, ≥98%
Stability And Storage
Store at -20℃ long term (24 months). Upon reconstitution, it is recommended to aliquot. Avoid freeze/thaw cycle. Store in the dark. Desiccated.
Storage
Protected from light, Store at -20°C, Desiccated, Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
This product requires cold chain shipping. Ground and other economy services are not available.
Grade
BioReagent
Purity
≥98%
Names and Identifiers
Molecular Weight
767.74
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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