Separation of T cells and B cells by magnetic bead method
This unit describes the separation of T cells, B cells, and T cell subpopulations by magnetic separation techniques. Author: J.E. Collier et al, Translator: Xui
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3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
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B Proliferation assays for cell function
Author: J.E. Colligan et al, Translator: Xuitao Cao et al, This experiment is from "Compendium Immunology Laboratory Guide".
Induction and detection of cytotoxic T lymphocytes
This unit describes several experimental protocols for inducing cytotoxic T lymphocytes and assaying their activity Remember that, in principle, any kind of cel
Cloning, Expression and Modification of Antibody Variable Regions (V Regions)
PCR-based cloning schemes utilize conserved sequences in the framework and pilot regions of the variable region, and although primers can be synthesized with co
Human α2a, α2b Interferon Assay
An indispensable part of the production process of recombinant human α2a/α2b interferon is the quality control of the product, and the bioactivity o
Establishment of T-cell clones
This unit provides a method for establishing specific T cell clones. Note that the key to the success of this method is the selection and preparation of conditi
Detection of lymphocyte migration and proliferation using the intracellular fluorescent dye CFSE
Author: J.E. Colligan et al, Translator: Xuitao Cao et al, This experiment is from "Compendium Immunology Laboratory Guide".
Hydrolysis of immunoglobulin G (IgG) fragments
When a new antibody needs to be fragmented, it is often necessary to perform a touch-and-go test. When choosing the reaction time, it is preferable to keep part
BrdU assays for d-cell and B-cell proliferation
Author: J.E. Colligan et al, Translator: Xuitao Cao et al, This experiment is from "Compendium Immunology Laboratory Guide".