Preparation of mouse T-cell hybrid tumors
T-cell hybridomas can be obtained by fusing activated T cells with tumor cells. Heterogeneous hybridomas can be cloned by limited dilution to obtain hybridomas
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3228 articles
Detection of tyrosine protein kinase by immunocomplex methods
The low abundance of tyrosine protein kinase (T P K ) expression and its rapid activation and inactivation make it difficult to purify using conventional bioche
Analysis of mitogen-activated protein kinase (MAPK) activity in T cells
Author: J.E. Colligan et al, Translator: Xuitao Cao et al, This experiment is from "Compendium Immunology Laboratory Guide".
Blotting assay for anti-phosphorylated tyrosine
Author: J.E. Colligan et al, Translator: Xuitao Cao et al, This experiment is from "Compendium Immunology Laboratory Guide".
Analysis of inositol phospholipid turnover during lymphocyte activation
Receptor-mediated activation of phospholipase C (P L C) induces hydrolysis of membrane inositol phospholipids to produce diacylglycerol (D A G) and water-solubl
Gamma interferon assay
Author: J.E. Colligan et al, Translator: Xuitao Cao et al, This experiment is from "Compendium Immunology Laboratory Guide".
Detection of IL-18
Newly synthesized IL-18 is a precursor molecule to the signal peptide, which is enzymatically cleaved in order to activate it. In this ELISA method, in order to
IL-10 detection assay
This protocol uses an antibody specific for mouse IL- 1 0 in the E LISA method to detect mouse IL- 1 0 , and does not detect human or viral IL-10. IL-10 from ot
IL-2 and IL-4 assays
A method is described for the detection and quantification of one of the cytokines in the presence of the other using neutralizing antibodies to murine IL-2 and