3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
This protocol is for the detection of cytokine production by activated antigen-specific T cells in vitro. If other stimulants or stimulated cells are used in vi
The ELISA method can be used to detect human IL-15 in culture supernatants and serum samples. This method can also be used for the detection of monkey IL-15, bu
ELISA is highly specific for human or mouse IL-13 and does not recognize other cytokines in the sample. Author: J.E. Colligan et al, Translator: Xuitao Cao et a
This is a method for the specific detection of IL-12 heterodimers. Since IL-12 p4 0 monomer cannot be detected, this method is able to detect IL-12 p7 5 heterod
Enzyme-linked immunospot test (ELISPOT) is based on the principle that cells that secrete certain protein molecules (e.g. cytokines) are able to secrete a relat
Mouse macrophage cell lines with tumor cell killing activity, such as A N A -1 and G G 2E E . Mouse primary macrophages can be freshly isolated. Author: J.E. Co
For granular antigens or liposome-encapsulated antigens, macrophages were applied as antigen-presenting cells. Author: J.E. Colligan et al, Translator: Xuitao C
An assay for macrophage phagocytosis and killing function. Author: J.E. Colligan et al, Translator: Xuitao Cao et al. This experiment is from "Compendium Immuno
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