QUALITÀ & PUREZZACarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.0.9 mg/mL
ExactAb™, Validated, Carrier Free, High performance, 0.9 mg/mL Carrier Free,ExactAb™,High Performance,Validated per flussi di lavoro cromatografici e analitici sensibili che richiedono un'interferenza minima della linea di base.
🌡
Stoccaggio e spedizione
Store at -20°C,Avoid repeated freezing and thawing Spedizione Ice chest + Ice pads. Consultare il COA specifico del lotto per le specifiche esatte.
📋
Documenti di qualità
SDS, COA, scheda tecnica e scheda delle specifiche disponibili per il download. COA specifico del lotto accessibile tramite ricerca per numero di lotto.
📚
Prova documentale
Citato in 0 pubblicazioni peer-reviewed su cromatografia, sintesi organica e reazioni di accoppiamento incrociato.
Panoramica
Rabbit anti Human ATP6V1D Antibody, Polyclonal, could be used for WB, IHC and so on. Application: WB: 1/500-1/4000 IHC: 1/50-1/100 Protein Function Relevance': ""ATP6V1D is a subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase is an heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c', c'' and d). It is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system.
Specifiche
Product Name
ATP6V1D Antibody - Anticorpo primario, specific to ATP6V1D, Rabbit IgG
Sinonimi
ATP6M antibody | ATP6V1D antibody | V-ATPase 28 kDa accessory proteinantibody | V-ATPase subunit D antibody | V-type proton ATPase subunit D antibody | Vacuolar ATP synthase subunit D antibody | Vacuolar proton pump subunit D antibody | VATD antibody | VM
Specifiche e purezza
ExactAb™, Validated, Carrier Free, High performance, 0.9 mg/mL
Specie ospite
Coniglio
Specificità
ATP6V1D
Immunogeno
Fusion protein of human ATP6V1D (AA 169-238).
Controllo positivo
WB: Mouse brain tissue lysate, NIH/3T3, C6, HeLa and HEK293 cell lysates. IHC: Human colorectal cancer tissue. IF/ICC: HeLa cells.
Coniugazione
Unconjugated
Grado
Carrier Free, ExactAb™, High Performance, Validated
Isotipo
Rabbit IgG
SDS-PAGE
150 kDa
Metodo di purificazione
Protein A purified
Concentrazione
0.9 mg/mL
Product Line
Prodotti biologici
Stoccaggio e spedizione
Forma
Liquid
Condizioni di conservazione di stoccaggio
Store at -20°C,Avoid repeated freezing and thawing
Spedito in
Ice chest + Ice pads
Stabilità e conservazione
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Immagini
ATP6V1D Antibody (Ab090177) - Western Blot All lanes: ATP6V1D Antibody (Ab090177) at 1/4000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 42 kDa Observed band size: 41 kDa Exposure time: 113.8 s
ATP6V1D Antibody (Ab090177) - Western Blot All lanes: ATP6V1D Antibody (Ab090177) at 1/4000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 42 kDa Observed band size: 41 kDa Exposure time: 17.7 s
ATP6V1D Antibody (Ab090177) - IHC Immunohistochemistry analysis of paraffin-embedded Human colorectal cancer using ATP6V1D Antibody (Ab090177). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
ATP6V1D Antibody (Ab090177) - IF/ICC
IF analysis of ATP6V1D Antibody (Ab090177) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with ATP6V1D Antibody (Ab090177) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
Applicazione
Applicazione
Dilution info
IHC
1/50 - 1/100
WB
1/500-1/4000
IF/ICC
1/200
Documentazione
📋 Scheda di dati di sicurezza (SDS)
Documento completo su pericoli, manipolazione, stoccaggio e conformità normativa.
Determine the necessary mass, volume, or concentration for preparing a solution.
Dilution Calculator
Determine the dilution needed to prepare a stock solution.
Reconstitution Calculator
Recensioni
Recensioni dei clienti
Hai bisogno di aiuto per scegliere la qualità?
La nostra guida alla selezione delle qualità copre purezza, stato dello stabilizzatore e idoneità all'applicazione per tutte le varianti del nostro catalogo.
Utilizziamo i cookie per garantire il corretto funzionamento del sito web e, ove consentito, per migliorare la tua esperienza. Puoi gestire le tue preferenze in qualsiasi momento nelle Impostazioni. Per saperne di più, consulta la nostra Cookie Policy.
Desidera che le inviamo un messaggio quando avremo sconti disponibili?
Ricordamelo più tardi
Grazie! Controlli la sua casella di posta per confermare.
Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
ZJ23F1202129