CD8 alpha Rat mAb (PE-Cy7) - Anticorpo primario, specific to CD8 alpha

Cat. No.: Ab190211

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

consiglio

Reactive species

Mouse

Isotype

Rat IgG2a

Clone Number

53-6.7

Conjugation

PE-Cy7

Panoramica

Rat anti Mouse CD8 alpha Antibody, Monoclonal (53-6.7), could be used for Flow Cytometry.

Specifications

Product Name

CD8 alpha Rat mAb (PE-Cy7) - Anticorpo primario, specific to CD8 alpha

Sinonimi

Ly-2 | Ly-35 | Ly-B | Lyt-2 | CD_antigen: CD8a | CD8 antigen, alpha polypeptide (p32) | CD8 | CD8a molecule | CD8A | Leu2 T-lymphocyte antigen | LEU2 | MAL | OKT8 T-cell antigen | p32 | T cell co-receptor | T8 T-cell antigen | T-cell antigen Leu2 | T-cell

Specifiche e purezza

ExactAb™, Validated, 0.5 mg/mL

Meccanismi biochimici e fisiologici

Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.

Specie ospite

consiglio

Specificità

CD8 alpha

Immunogeno

Mouse Spleen Cells or Thymocyte Membranes

Controllo positivo

Flow Cytometry: C57BL/6 mouse splenocytes

Coniugazione

PE-Cy7

Eccitazione (nm)

Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)

Emissione (nm)

774nm

Ex/Em(nm)

Ex:565nm;Em:774nm

Grado

ExactAb™, Validated

Tipo di catena leggera

kappa

Metodo di purificazione

Protein G purified

Concentrazione

0.5 mg/mL

Stoccaggio e spedizione

Condizioni di conservazione di stoccaggio

Store at 2-8°C,Protected from light,Do not freeze

Spedito in

Wet ice,Do not freeze

Stabilità e conservazione

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Immagini

Flow Cytometry: CD8 alpha Rat mAb (PE-Cy7) (Ab190211)

CD8 alpha Rat mAb (PE-Cy7) (Ab190211) - Flow Cytometry
Flow cytometry analysis of CD8 alpha expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). C57BL/6 mouse splenocytes cells were preincubated with CD16/CD32 Rat mAb (A412018). The cells were then stained with CD8 alpha Rat mAb (PE-Cy7) (Ab190211) at 0.1μg/test for 1 hour at 4°C. Left panel - Rat IgG (PE-Cy7) (Ab190073).

Flow Cytometry: CD8 alpha Rat mAb (PE-Cy7) (Ab190211)

CD8 alpha Rat mAb (PE-Cy7) (Ab190211) - Flow Cytometry
Flow cytometry analysis of CD8 alpha expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). C57BL/6 mouse splenocytes cells were preincubated with CD16/CD32 Rat mAb (A412018). The cells were then stained with CD8 alpha Rat mAb (PE-Cy7) (Ab190211) at 0.1μg/test for 1 hour at 4°C. Blue - Rat IgG (PE-Cy7) (Ab190073). Black - Unlabelled control, cells without incubation with primary antibody.

Applicazione

• Applicazione: Dilution info
• Flow Cytometry:

  ≤0.3μg/test

  For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.;

Obiettivi associati (umani)

CD8A Tbio T-cell surface glycoprotein CD8 alpha chain (0 Activities)

Discover Target: CD8A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)