Recombinant CD8 alpha Antibody (Biotin) - Primary antibody, specific to Mouse CD8α

Cat. No.: Ab213871

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

Rat

Reactive species

Mouse

Isotype

Rat IgG2a

Clone Number

P83E51

Conjugation

Biotin

Overview

Recombinant CD8α Antibody, clone P83E51, could be used for Flow Cytometry.

Specifications

Product Name

Recombinant CD8 alpha Antibody (Biotin) - Primary antibody, specific to Mouse CD8α

Synonyms

alpha polypeptide (p32) antibody | CD_antigen=CD8a antibody | CD8 antibody | CD8 antigen alpha polypeptide antibody | CD8 antigen alpha polypeptide (p32) antibody | CD8 antigen, alpha polypeptide (p32) antibody | CD8a antibody | CD8A antigen antibody | CD

Specifications & Purity

ExactAb™, Validated, 0.5 mg/mL

Biochemical and Physiological Mechanisms

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide com

Host species

Rat

Specificity

Mouse CD8α

Immunogen

Mouse Spleen Cells or Thymocyte Membranes

Positive Control

Flow Cytometry: C57BL/6 mouse splenocytes

Conjugation

Biotin

Grade

ExactAb™, Validated

Light Chain Type

kappa

Purification Method

Protein A purified

Concentration

0.5 mg/mL

Storage and Shipping

Storage

Store at 2-8°C,Protected from light,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

Flow Cytometry: Recombinant CD8 alpha Antibody (Biotin) (Ab213871)

Recombinant CD8 alpha Antibody (Biotin) (Ab213871) - Flow Cytometry
Flow cytometry analysis of CD8 alpha expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were stained with Recombinant CD8 alpha Antibody (Biotin) (Ab213871) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Left panel - Rat IgG (Biotin) (Ab180310).

Flow Cytometry: Recombinant CD8 alpha Antibody (Biotin) (Ab213871)

Recombinant CD8 alpha Antibody (Biotin) (Ab213871) - Flow Cytometry
Flow cytometry analysis of CD8 alpha expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with Recombinant CD8 alpha Antibody (Biotin) (Ab213871) 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Rat IgG (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• Flow Cytometry:

  ≤0.3μg/test

  For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.;

Associated Targets(Human)

CD8A Tbio T-cell surface glycoprotein CD8 alpha chain (0 Activities)

Discover Target: CD8A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)