DNA Agarose Nucleic Acid Electrophoresis
Nucleic acid electrophoresis is an important tool for conducting nucleic acid research and can be used for techniques such as (1) nucleic acid probing; (2) nucl
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Laboratory Protocols & SOPs Library
3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
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Experiments on DNA precipitation
The experiment of precipitating DNA can be applied to isolate DNA.
DNA blotting and narrow line blotting experiments
Spot and narrow line blotting experiments that can be used to detect the relative abundance of target sequences in blotted 1DNA products. The main applications
Genomic DNA preparation in mammals
Various factors that break and degrade DNA should be avoided as much as possible during DNA extraction to ensure the integrity of DNA and lay the foundation for
DNA blot hybridization analysis experiment
Hybridization analysis is based on the principle that a single-stranded DNA molecule of a specific sequence (i.e., a commonly labeled "probe") can form a base p
Freeze-thaw method for recovery of DNA fragments
The freeze-thaw method of recovering DNA fragments can be used to (1) obtain purified DNA fragments and (2) recover PCR products.
Gene transfection (calcium phosphate-DNA co-precipitation)
Gene transfection technology can be applied to: genome function research (gene expression regulation, gene function, signal transduction and drug screening rese
Construction of recombinant DNA by polymerase chain reaction
The polymerase chain reaction constructs recombinant DNA for a wide range of uses (1) for sequencing primers (2) targeted mutagenesis (3) nucleic acid hybridiza
Determination of DNA by chemical method - Diphenylamine colorimetric method
Chemical determination of DNA content can be used for (1) estimation of plasmid concentration prior to transformation and transfection, and (2) assay of acquire