Determination of DNA by chemical method - Diphenylamine colorimetric method
Chemical determination of DNA content can be used for (1) estimation of plasmid concentration prior to transformation and transfection, and (2) assay of acquire
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Analysis of radioisotope-labeled DNA sequence determination
Radioisotope-labeled DNA sequencing analysis can be applied to analyze gene structure-function relationships.
Gene transfection (calcium phosphate-DNA co-precipitation)
Gene transfection technology can be applied to: genome function research (gene expression regulation, gene function, signal transduction and drug screening rese
DNA blot hybridization analysis experiment
Hybridization analysis is based on the principle that a single-stranded DNA molecule of a specific sequence (i.e., a commonly labeled "probe") can form a base p
Plasmid DNA purification by polyethylene glycol precipitation assay
This method was first devised by Richaed Treisman (ICRF, London, UK) with reference to the work of Lis, who was the first to use polyethylene glycol (PEG) to is
Removal of ethidium bromide from DNA by ion exchange chromatography
Ethidium bromide in DNA purified by cesium chloride gradient centrifugation can be removed by ion-exchange chromatography, followed by ethanol precipitation to
Removal of small fragments of nucleic acids from plasmid DNA preparations by NaCl centrifugation
Conventional plasmid DNA preparations are contaminated, to varying degrees, with small molecules of DNA or RNA from the genome of the host bacterium or plasmid
Plasmid DNA preparation by the toothpick method in small quantities
Colonies picked with a toothpick from agar medium can be used directly to prepare plasmid DNA. the resulting closed-loop DNA often has too many impurities to be
Removal of ethidium bromide from DNA by extraction with organic solvents
To remove ethidium bromide from CsCl-gradient purified DNA, repeated extraction with organic solvents is commonly used. Subsequently, CsCl can be removed by dia