Pyrophosphate sequencing
Pyrosequencing is a luminescence-based sequencing technology for the determination of pyrophosphate (PPi). The three key points of pyrosequencing are: (1) dATPa
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Laboratory Protocols & SOPs Library
3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
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Mutagenesis of genomes using chemical mutagens
Currently, there are two main strategies for studying gene function using model mice: the gene-driven approach and the phenotype-driven approach. Gene knockout
Automated Laser Fluorescence DNA Sequencing
At present, the application of automated laser fluorescence DNA sequencing technology (also known as first generation sequencing technology) has been very commo
Agarose gel electrophoresis experiment
Agarose gel electrophoresis can be applied to (1) DNA cutting and recovery, (2) DNA isolation, (3) corroboration of whether DNA is recombinant or not, whether p
Cross-protection neutralization experiments
When an animal is infected by a virus, specific neutralizing antibodies are produced in the body and bind specifically to the corresponding virus particles, thu
Speckled gold immunofiltration assay (DIGFA) assay
The spotted gold immunofiltration assay (DIGFA) is available as a clinical test.
Antigen-specific T cell clone preparation assay
The antigen-specific T cell clone preparation assay can be used to: prepare antigen-specific T cell clones.
Polyclonal antibody purification assay
The octanoic acid extraction method can be used to isolate and extract Ig from human, rabbit and mouse serum and McAb from hybridoma-induced ascites and culture
Radioimmuno-convection electrophoresis assay
When an antibody (or antigen) labeled with a radioactive element is precipitated with the corresponding antigen (or antibody), the precipitation line is confirm