MTT (tetrazolium salt) colorimetric method
The tetrazolium salt (MTT) colorimetric assay can be applied to (1) detection of cell survival and growth, (2) large-scale screening of antitumor drugs, (3) cyt
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3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
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Enzyme immunoelectrophoresis
Enzyme immunoelectrophoresis can be applied to the analysis and identification of enzyme proteins.
Insulin assay experiment
Radioimmunoassay of insulin can be used for (1) immunolabeling detection techniques using radioisotopes as tracers, and (2) competitive binding of isotope-label
Polyclonal Antibody Preparation Experiment
Antigens are usually composed of multiple antigenic determinants, and the antibody produced by a B-lymphocyte receiving an antigen stimulated by one antigenic d
Identification and characterization experiments of human tumor antigens
Human tumor antigens can be (1) used for tumor diagnosis and (2) immunotherapy.
ELISPOT
With the wide application of enzyme-linked immunoassay (ELISA) technology in the field of medicine and biology, new breakthroughs have been made in the study of
Tumor protein vaccine prophylactic trials
Tumor protein vaccine prophylactic trials are designed to examine whether the vaccine's targets are directed against a wide range of antigens, can cover 90% or
immunohistochemical staining
Immunohistochemical staining refers to the combination of fluorescent or colorable chemicals on antibodies to detect the presence of target antigens in cells or
Serological selection for cloning of new antigens or genes
E.coli/ phage lysate adsorption serum removes antibodies in serum that immunoreact with bacterial and phage lysin, this reduces the false positive rate, the ser